以PCL-PEG为载体的人参皂苷Rg3纳米粒的制备及表征.pdfVIP

第47卷第3期 应 用 化 工 Vol.47 No.3 2018年3月 Applied Chemical Industry Mar.2018 以PCL-PEG为载体的人参皂苷 Rg3 纳米粒的制备及表征 1 1 1 2 2 苏晓梅 ,张丹参 ,赵凯燕 ,张海威 ,侯文书 (1.河北科技大学 化学与制药工程学院,河北 石家庄 050000;2.河北北方学院药学系,河北 张家口 075000) 摘 要:以PCL-PEG为载体,采用乳化溶剂蒸发法制备人参皂苷Rg3纳米粒,以包封率和载药量为指标,通过正交 实验优化制备条件。 结果表明,最优制备条件为:人参皂苷Rg3浓度为3mg/ mL,PCL-PEG浓度为5mg/ mL,胆酸钠 的体积为5 mL,胆酸钠浓度为0.5%。 得到的纳米粒为球形,表面圆整光滑,粒径为129.1 nm,电位为-38.7 mV, 包封率为90.03%,载药量为34.18%,稳定性良好,具有明显的缓释性能。 关键词:人参皂苷Rg3;PCL-PEG;纳米粒;制备;表征 中图分类号:TQ461 文献标识码:A 文章编号:1671-3206(2018)03-0525-05 Preparation and characterization of ginsenoside Rg3 nanoparticles based on PCL-PEG 1 1 1 2 2 SUXiao-mei ,ZHANGDan-shen ,ZHAOKai-yan ,ZHANGHai-wei ,HOU Wen-shu (1.College of Chemisty and Pharmaceutical Engineering,Hebei University of Science and Technology, Shijiazhuang050000,China;2.Department of Pharmacology,Hebei North University,Zhangjiakou075000,China) Abstract:Take PCL-PEGasthe vector,the ginsenoside Rg3 nanoparticleswere prepared using the emul- sion solventevaporationtechnique,andthepreparationprotocolwasinvestigatedontheresponseof encap- sulation ratio and drug-loading coefficient,the preparation condition was optimized by orthogonal experi- mental design.The results showed that the optimum conditions as following:concentrations of Rg3 and PCL-PEG were 3 mg/ mL and 5 mg/ mL,respectively.The volume of sodium cholate was5 mL,the con- centration of sodium cholate is 0.5%.The shape of nanoparticle was spherical,the surface was regular and smooth;the size was 129. 1 nm,the zeta potentia