二维相关红外光谱研究再生蚕丝蛋白膜的构象与温度之间的-复旦大学.PDF

2004 年第 62 卷 化 学 学 报 Vol. 62 , 2004 第 21 期 , 2127～2130 ACTA CHIMICA SINICA No. 21 , 2127 ～2130 ·研究通讯· 二维相关红外光谱研究再生蚕丝蛋白膜的构象与温度之间的关系 彭显能　　陈　新　　武培怡　　邵正中 (复旦大学高分子科学系 　教育部聚合物分子工程重点实验室 　上海 200433) 摘要 　通过二维相关红外光谱 ,研究了再生蚕丝蛋白膜的构象及其转变与温度之间的关系. 实验结果表明 ,将样品从 130 ℃升温到220 ℃、或在 180 ℃的恒温过程中 ,丝素蛋白分子链的构象会发生变化 ,且不同构象对温度升高过程或 180 ℃恒温 β α 过程响应的顺序是无规线团变化先于 折叠、螺旋的形成. 关键词 　二维相关红外光谱 ,桑蚕 ,丝蛋白 ,薄膜 Investigation on the Conformation Transition of Regenerated Silk Fibroin Films under Thermal Treatment by twodimensional ( 2D) correlation FTIR spectroscopy PENG, XianNeng 　　CHEN , Xin 　　WU , PeiYi 　　SHAO , ZhengZhong ( ) Department of Macromolecular Science , The Key Laboratory of Molecular Engineering of Polymers , Fudan University , Shanghai 200433 Abstract 　With the advantage of twodimensional (2D) correlation FTIR spectroscopy , the conformation transition of regenerated silk fibroin film was studied under thermal treatment. The results showed that the conformation changed when the film was heated from 130 to 220 ℃as well as maintained at 180 ℃for different time courses. It was also found that the conformation response order was random coil prior to β α sheet and helix. Keywords 　twodimensional correlation FTIR spectroscopy , Bombyx