九十年生物医学工程科技研讨会论文.doc

PAGE PAGE 3 特定結合分子固定化表面對於內毒素吸附行為之評估 An assessment on specific-binding ligand immobilized surface for endotoxin adsorption 陳秀綾老師 國立瑞芳高級工業職業學校 中文摘要 本研究擬探討不同特定結合分子固定化表面與細菌內毒素彼此間特異性吸附的行為。研究中將探討普遍使用於敗血症治療時所使用的抗生素與內毒素分子之作用，利用表面電漿共振(SPR)之流動吸附系統線上偵測其彼此間專一性動態吸附行為, 並將吸附實驗所得結果進行與理論模型分析，進一步計算平衡與動態參數。SPR晶片表面將搭配以橢圓儀、接觸角量測儀測定表面膜厚及親疏水性質、FTIR或ESCA量測修飾表面之官能基與分佈狀況來進行固定化表面之定性測試,及評估不同抗生素對於移除細菌內毒素之效能評估。初步測試SPR系統進行蛋白質吸附金面的清洗實驗，結果確定以0.5% SDS界面活性清洗劑流過晶面並無法徹底清除吸附在金面之HAS分子。未來工作將進行內毒素與抗生素固定化表面分別對於對應分子間特異性吸附之行為進行研究探討，進而評估篩選較適合用於作為發展體外血液灌注處理器之表面固定化抗生素分子。 關鍵詞：內毒素；特異性吸附；表面修飾；抗生素 Abstract Various specific-binding ligand immobilized surfaces for the performance of endotoxin adsorption will be evaluated in this study. The interaction between endotoxin and antibiotics generally used in treating sepsis will be studied. A flow system with Surface Plasmon Resonance (SPR) is used to monitor the dynamic behavior of this specific adsorption. Experimental data of SPR are fitted to theoretical model to determine the equilibrium binding, dynamic dissociation, and association constants. Accompanied with the thickness measurement of binding molecules via ellipsometry, hydrophilicity characterization by contact angle measurement, and the presence of specific functional group or chemical compositions by FTIR or ESCA, the capability of specific antibiotics on the removal of endotoxin are evaluated. Preliminary tests focused on evaluating cleanness methods for Human Albumin adsorbed surfaces and found that by purging with a 0.5% SDS surfactant solution cannot thoroughly remove the adsorbed protein from the surface. In the next, we shall study the adsorption behavior of antibiotics to endotoxin immobilized surface, and the adsorption behaviors of endotoxin to antibiotics immobilized surface as well. The specific binding behaviors between endotoxin with preferred antibiotics shall be further evaluated for the development of an antibiotics immobilized extracorporeal hemoperfusion device. Keywords: endotoxin,