大鼠在体心肌缺血再灌注损伤组织间液钙离子动态与.doc

大鼠在体心肌缺血再灌注损伤组织间液钙离子动态变化与 胞膜钙转运ATP酶表达 黄邵洪1，荣健2，王淑云3，吴钟凯2 (510630广州，中山大学附属第三医院胸心外科1，510080广州，中山大学附属第一医院心脏外科2，510630广州，中山大学附属第三医院急诊科3) [摘 要] 目的 检测大鼠心肌在体缺血再灌注损伤（IR）组织间液钙离子浓度，比较IR前后心肌胞膜钙转运ATP酶（PMCA）mRNA表达水平，探讨其对钙超载的意义。方法 12只SD大鼠随机分为IR和对照组（6只/组），以微透析技术连续收集心肌组织间液标本，原子吸收光谱法检测钙离子浓度。IR组通过结扎（20min）后松解（60min）前降支造成心肌缺血再灌注，结束时获取收取左心室缺血区和右心室心肌行PMCA mRNA定量PCR检测，实验前后采血检测血钙和肌钙蛋白T。对照组免除结扎松解前降支，其余操作与IR组一致。结果 IR组血浆cTnT浓度显著升高（control vs IR，ng/mL：1.62±0.6 vs 4.29±2.22，p=0.031）。IR组心肌组织间液钙离子浓度在缺血期无变化，再灌注20min显著下降，心肌析出液钙离子浓度与对照组存在显著性差异（control vs IR，ng/mL：224±31 vs 136±39，p=0.002）。血浆Ca2+浓度实验前后无显著差异。心肌细胞膜PMCA mRNA表达水平在组间和组内均无显著差异。结论 大鼠在体心肌缺血20min再灌注60min，组织间液钙离子浓度在缺血期无明显变化，再灌注期迅速下降，这种变化趋势与胞膜PMCA表达无关。 [关键词] 缺血再灌注损伤；钙离子；胞膜钙转运ATP酶 [中图分类号] R363 [文献标识码] A [基金项目] 国家重点基础研究发展计划资助项目（2010CB529500）, 中山大学5010计划项目（2007008） [通信作者] 吴钟凯，电话E-mail： HYPERLINK mailto:jiji126@126.com jiji126@126.com Change of Interstitial Calcium and Expression of Sarcolemal PMCA Expressing in Rat Myocardial Ischemia Reperfusion Myocardium Injury in vivo Huang Shaohong1, Rong jian2, Wang Shuyun3, Wu Zhongkai2(1Department of Thoracocardiac Surgery, The Third Affiliated Hospital, Sun Yet-sen University, Guangzhou, 510630, China. 2Department of Cardiac Surgery, The First Affiliated Hospital, Sun Yet-sen University, Guangzhou 510080, China. 3Department of Emergency, The Third Affiliated Hospital, Sun Yet-sen University, Guangzhou, 510630, China.) [Abstract] Objectives To detect the dynamics of intersitial calcium and the expression of sarcolemal PMCA mRNA expressing in rat myocardial ischemia reperfusion （IR）myocardiuminjury（IR） in vivo. Methods Twelve SD rats were randomized into IR or control groups(n=6). The Mmyocardial interstitial fluid was collected continously with microdialysis technique and the calcium concentrations wereas measured with an atomic absorption spectrophotometer. In IR group, LAD(补前降支英文全称) wereas ligated for 20 mins then released for 60 mins to achieved IR effects. The left ventricle(i