采后竹笋木质化与内源激素的关系.PDF

维普资讯 中国农业科学 2006，39(4)：792—797 ScientiaA~riculturaSinica 采后竹笋木质化与内源激素的关系 罗 自生 (浙江大学食品科技系，杭州 310029) 摘要：【目的】研究采后竹笋贮藏期间的木质化机理，探讨采后竹笋内源激素对木质化进程的调控作用。 【方 法】以采收后雷竹笋和毛竹笋为材料，分析了两个品种竹笋在 (2±1)。C下贮藏期间内源激素 (IAA、GA3、ABA) 含量和木质素合成相关酶活性的动态变化规律。【结果】雷竹笋和毛竹笋贮藏期间，GA，和 IAA含量都逐渐减少， 而ABA含量先增加后减少．其中雷竹笋的GA，、IAA和ABA含量高于毛竹笋的。两个品种竹笋的苯丙氨酸解氨酶 (PAL)、 4一香豆酸辅酶A连接酶(4eL)和过氧化物酶 (POD)活性都先增加后减少，木质素和纤维素含量逐渐增加，导致竹 笋组织硬度增加。经相关分析发现，两个品种竹笋的PAL、4eL和POD活性与ABA／GA，的比值都呈显著正相关。【结 论】ABA／GA，比值的变化对竹笋木质化起着关键的调控作用，推测通过降低ABA／GA，比值可抑制PAL、4eL和POD活 性而延缓竹笋的木质化进程。 关键词：竹笋；木质化；内源激素 TheRelationshipBetweenLignificationandEndogenousHormone ContentofExcisedBambooShoots LUO Zi-sheng (DepartmentofFoodScienceandTechnology,ZhefiangUniversity,Hangzhou310029) Abstract： 【0biective】Inordertostudythemechanismoflignificationnadresearchontheimpactsofendogenoushormone (IAA，GA3，ABA)onlignificationinexcisedbambooshoots．[Method]Therelationshipbetweenendogenoushomr one(IAA，GA3， ABA)levelsnad enzymeactivitiesrelated tolignificationofPhyllostachyspraecoxfpreveynalisnadPhyllostachyspubescens Mazelwereinvestigated． [Result]ThecontentofGA3andIAAinhtetwobambooshootsdeclinedcontinuously,butABAcontent increased initiallynaddeclinde later~Thecontentofendogenoushomrone(IAA，GA3，ABA)in excisde bma boo shootsof Phyllostachyspraecoxf．preveynaliswerehigherhtna thoseofPhyllostachyspubescensMaze1．Theactivitiesofphenylalnaine ammonialyase(PAL)，4-coumraateCoAligase(4eL)nadperoxidase(POD)increasedinitiallynaddeclinde laterduringhtestorage． Th econtentofligninna dcellulosenadfirmnessofbma booshootscontinuouslyincreased．Th eactivitiesof眦 ．4eLna dPOD were significnatlycorrelatedwiht hteratioofABA／GA3． [Conclusion