依托咪酯对成年大鼠脊髓胶状质局部突触传递的作用-生理学报.PDFVIP

生理学报 Acta Physiologica Sinica, June 25, 2004, 56 (3): 413-418 413 李 震等: 依托咪酯对成年大鼠脊髓胶状质局部突触传递的作用效果 研究论文 依托咪酯对成年大鼠脊髓胶状质局部突触传递的作用 李震, 罗层，孙焱芫，陈军* 第四军医大学全军神经科学研究所, 西安 710032 摘 要: 应用盲插全细胞膜片钳技术, 在成年大鼠脊髓薄片上观察依托咪酯(etomidate, ET)对脊髓胶状质局部突触传递的影响。实 验结果显示, 在钳制电压为-70 mV 时, 500 µmol/L 的ET 对微小兴奋性突触后电流(mEPSC)的持续时间、频率和幅度都无明显的 作用。在钳制电压为0 mV 时, 50 µmol/L 的ET 使 GABA 能微小抑制性突触后电流(mIPSC)的持续时间延长45.57 ±12.46% (P 0.05), 但对其频率和幅度无影响。同样在钳制电压为 0 mV 的情况下, 50 µmol/L 的ET 对甘氨酸能mIPSC 的持续时间、频率 及幅度均无作用。以上结果表明, 在成年大鼠的脊髓胶状质, ET 主要通过延长 GABA 能mIPSC 的持续时间, 即延长受体通道 的开放时间发挥作用, ET 对于兴奋性的突触传递没有直接的作用。 关键词: 依托咪酯; 脊髓; 胶状质; 突触传递 中图分类号: Q426; R971 Effects of etomidate on local synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord LI Zhen, LUO Ceng, SUN Yan-Yan , CHEN Jun* Institute of Neuroscience, Fourth Military Medical University, Xi ’an 710032, China Abstract: By using blind spinal slice whole-cell patch-clamp technique, we observed the influence of etomidate (ET) on synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord. Male adult Sprague-Dawley rats (7~8 weeks old) were anaesthetized with urethane (1.2 g/kg, i.p.), and then lumbosacral laminectomy was performed. The lumbosacral spinal cord (L1~S3) was removed and placed in preoxygenated Krebs solution at 1~3ºC. After cutting all of the ventral and dorsal roots, the pia-arachnoid membrane was removed. The spinal cord was mounted on a vibrating microslicer and then a 500 µm thick transverse slice was cut. The slice was placed on a nylon mesh in the recording chamber, and then perfused at a rate of 15~20 ml/min with Krebs solution saturated with 95% O and 5% CO , and maintained at 36 2 2 ±1ºC. Substantia gelatinosa neurons were identified by their location. Under a binocul