尼古丁是脂肪酶的反竞争抑止剂的试验研究.PDFVIP

2004 年第 62 卷 化 学 学 报 Vol. 62 , 2004 第 10 期 , 1003～1006 ACTA CHIMICA SINICA No. 10 , 1003 ～1006 ·研究简报· 尼古丁是脂肪酶的反竞争抑止剂的实验研究 林 a b a a , a 　琳 　　陈锡侨 　　赵福群 　　唐应武 　　张复实 ( a 清华大学化学系 　有机光电子与分子工程教育部重点实验室 　北京 100084) ( b 澳门大学科技学院 　澳门 邮政信箱 3001 号) 摘要 　希望明确尼古丁对脂肪酶活性是否有抑止作用 ,用于解释医学已有统计数据 :吸烟的人体重普遍较轻 ,而戒烟后体 重会有回升. 用三油酸甘油酯为底物 ,在加入尼古丁前后 ,检测脂肪酶活性变化. 结果表明 :脂肪酶在人体外仍有活性 ,而且 尼古丁是脂肪酶反竞争性抑止剂. 在脂肪酶浓度为 7 mg/ mL 时 ,抑止程度达 50 %. 关键词 　脂肪酶 ,尼古丁 ,三油酸甘油酯 ,油酸 Study of Nicotine Acting as an Uncompetitive Inhibitor of Lipase LIN , Lin a 　　CHAN , ShekKiu b 　　ZHAO , FuQun a 　　TANG, YingWu a 　　ZHANG, FuShi , a ( a Key Lab of Organic Optoelectronic Molecular Engineering , Ministry of Education Department of Chemistry , Tsinghua University , Beijing 100084) ( b Department of Science and Technology , University of Macau , Macau) Abstract 　The aim of the study is to determine the role of nicotine as a lipase inhibitor in fat and fatty acid metabolism and to explain the clinic phenomenon : smokers weigh less than agematched nonsmokers , and most of smokers gain weight after smoking cessation. Lipase activity was measured in the presence or absence of nicotine using olein as substrate. Lipase is active in vitro . Nicotine is demonstrated to be an uncompetitive inhibitor of lipase at biologically reachable concentration. I