大头艾纳香对萝卜蚜的生物活性分析.pdfVIP

Studies on Bioactivity of Blumea megacephala Chang et Tseng against Lipaphis erysimi Kaltenbach M.D.candidate:Liu Yan-hua Supervior:Deng Ye-cheng Major:Ecology Research Direction: Applied Ecology Grade:2005 ABSTRACT One hundred seventeen species of plants collected from Guangxi zhuang autonomous region were extracted with methanol. Insecticidal activities of these extracts against Lipaphis erysimi Kaltenbach were determined by topical application. According to the insecticidal activity, Blumea megacephala Chang et Tseng, which had high contact activity against Lipaphis erysimi Kaltenbach, was selected to be further investigated for active ingredients. And the extract was separated by liquid -liquid extraction and purified through column chromatograghy. Primary mechanism was investigated through the change of several enzymes. The contact activities of 122 methanol extracts of 117 plants against Lipaphis erysimi Kaltenbach were determined by topical application in laboratory. Results showed that 2 plant extracts had high contact activity, with a corrected mortality of ＞80% at 10µg/larvae after 24 hours, they were that of Blumea megacephala Chang et Tseng, Pilea cavaleriei Levl. 15 plant extracts had medium activity, their corrected mortality ranging from 50% to 80%, they were that of Osbeckia crinita Benth. In Wall., Gnetum montanum Markgr, Evodia lepta Merr., Abrus precatorius L., Toxicodendron Succedaneum(Linn.), Mirabilis jalapa Lim., Croton cavaleriei Gagnep, Cibotium barometz(Linn.)J.Sm., Litsea cuheha(Lour.)Pers., Diplocyclos palmatus(L.)C., Stephania cepharantha Hayata, Curcr