基于生物质谱的胶原蛋白分析方法研究与应用-农产品加工及贮藏工程专业论文.docxVIP

万方数据 万方数据 关于学位论文原创性和使用授权的声明 本人所呈交的学位论文，是在导师指导下，独立进行科学研究所取得 的成果。对在论文研究期间给予指导、帮助和做出重要贡献的个人或集 体，均在文中明确说明。本声明的法律责任由本人承担。 本人完全了解山东农业大学有关保留和使用学位论文的规定，同意学 校保留和按要求向国家有关部门或机构送交论文纸质本和电子版，允许论 文被查阅和借阅。本人授权山东农业大学可以将本学位论文的全部或部分 内容编入有关数据库进行检索，可以采用影印、缩印或其他复制手段保存 论文和汇编本学位论文，同时授权中国科学技术信息研究所将本学位论文 收录到《中国学位论文全文数据库》，并向社会公众提供信息服务。 保密论文在解密后应遵守此规定。 论文作者签名： 导 师 签 名： 日 期： 符 号 说 明 HPLC：high performance liquid chromatography， 高效液相色谱 HPLC-MS：high performance liquid chromatography mass spectrum，液相色谱质谱联用 APCI：atmospheric-Pressure Chemical Ionization，大气压电离质谱 API：atmospheric-Pressure Ionization，离子喷雾电离质谱 ESI：electrospray ionization，电喷雾离子源 SIM: Selected ion monitoring, 选择离子检测 ACN：acetonitrile，乙腈 ECM：extracellular matrixc，细胞外基质 DNFB：2,4-dinitrofluorobenzene，2,4-二硝基氟苯 SDS：polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 TFA：Trifluoroacetic acid，三氟乙酸 Hyp：Hydroxyproline，羟脯氨酸 Pro：Proline，脯氨酸 Lys：Lysine，赖氨酸 Hyl：Hydroxylysine，羟赖氨酸 Ala：Alanine，丙氨酸 Phe：Phenylalanine，苯丙氨酸 Trp：Tryptophan，色氨酸 Tyr：Tyrosine，酪氨酸 Met：Methionine，甲硫氨酸 Cys：Cystine ，半胱氨酸 Glu：Glutamic acid，谷氨酸 Asp：Aspartic acid，天冬氨酸 Val：Valine，缬氨酸 Ser：Serine，丝氨酸 His：Histidine，组氨酸 Thr：Threonine，苏氨酸 FN：fibronectin，纤维连接蛋白 目 录 中文摘要 ·······························································································Ⅰ 英文摘要 ·······························································································Ⅲ 1 前言 ···································································································· 1 1.1 胶原蛋白概况 ······················································································ 1 1.1.1 胶原蛋白分子结构、类型及分布···························································· 1 1.1.2 胶原蛋白体内合成 ············································································· 3 1.2 胶原蛋白性能 ······················································································ 3 1.2.1 胶原蛋白生物学性能··········································································· 4 1.2.2 胶原蛋白生理化学性能················································