肠道菌群多样性在喂养不耐受新生儿中的作用.docVIP

肠道菌群多样性在喂养不耐受新生儿中的作用 唐小丽，余加林，艾 青，卢虹旭，李 阳，王政力，杨静丽，贺 雨，潘 云（400010 与英文不符重庆，重庆医科大学附属儿童医院新生儿中心，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室，重庆市儿童发育重大疾病诊治与预防国际科技合作基地） 与英文不符 【摘要】 目的 探讨肠道菌群多样性变化在喂养不耐受新生儿中的作用及其与疾病转归的关系。 方法 采用16S rDNA PCR联合变性梯度凝胶电泳（denatured gradient gel electrophoresis, DGGE）技术，分别对12例喂养不耐受新生儿（喂养不耐受组）发生喂养不耐受24 h内（t0）及喂养不耐受恢复后（t1）的粪便标本进行菌群多样性分析，并通过T-A克隆测序，了解其细菌种类分布及常见优势菌。同期采集12例孕周、出生体质量、日龄匹配的喂养耐受新生儿（对照组）粪便标本进行对比分析。结果 经DGGE分析，喂养不耐受新生儿粪便标本的条带丰富度较对照组显著下降（9.00±2.83 vs 12.76±2.38，P ＜ 0.05），香农威纳指数较对照组显著下降（2.12±0.36 vs 2.51±0.19，P ＜0.05）；喂养不耐受恢复后（t1）粪便标本条带丰富度、香农威纳指数与对照组比较，差异无统计学意义（14.00±1.91 vs 14.75±1.76, 2.61±0.14 vs 2.66±0.13,P ＞0.05）；克隆测序发现，喂养不耐受组粪便标本中克雷伯菌属比例较对照组增加(53.40% vs 40.94%，P＜0.05)。结论 喂养不耐受新生儿肠道菌群多样性降低，随着病情的恢复，肠道菌群多样性恢复；新生儿发生喂养不耐受可能与其肠道菌群中克雷伯菌属比例增加有一定相关性。 【关键词】 新生儿；喂养不耐受；肠道菌群；生物多样性 【中图法分类号】 R378；R722 【文献标志码】A Intestinal microflora community diversity in newborns with feeding intolerance Tang Xiaoli, Yu Jialin, Ai Qing, Lu Hongxu, Li Yang, Wang Zhengli, Yang Jingli, He Yu, Pan Yun (Department of Neonatology, Childrens Hospital of Chongqing Medical University; Ministry of Education Key Laboratory of Child Development and Disorders; Key Laboratory of Pediatrics in Chongqing; Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, China) 【Abstract】 HYPERLINK javascript:void(0); Objective To investigate the alterations of intestinal microflora community diversity in newborns with feeding intolerance (FI) and the relationship with disease outcome. Methods Using 16s rDNA PCR-DGGE, we assessed 12 FI newborns’ bacterial diversity in stool specimens within 24 hours of the symptom onset (t0) and after the recovery of FI (t1). T-A cloning kit and sequencing were used to investigate the distribution of bacteria and common dominant bacteria in stool specimens. At the same time, we collected the stool specimens of 12 feeding tolerance newborns with the same gestational age, birth weight and day-old as c