所需突变株信息.docxVIP

JT9609线虫株 Strain JT9609 Genotype pdk-1(sa680) X. Contains Gene: HYPERLINK /db/gene/gene?name=WBGenclass=Gene pdk-1 Variation: HYPERLINK /db/gene/variation?name=WBVaclass=Variation sa680 Properties Species: Caenorhabditis elegans Outcrossed: x4 Mutagen: EMS Received at CGC: 27 Aug 1999 Location HYPERLINK /CGC/ \t _blank CGC Made by HYPERLINK /db/misc/person?name=WBPerson9 Michael Ailion Remark Daf-c at 25C. Egl, Clumpy, Lon, low brood size. Dauers do not recover when moved to 15C in the presence of food. Daf-c, Lon, Clumpy and fertility defects can be rescued maternally. Maintain at 15C. Reference 1999: HYPERLINK /db/misc/paper?name=WBPapeclass=Paper Paradis S et al.: 1999. A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans. Genes Dev 13: 1438-52. Description: pdk-1 encodes the C. elegans 3-phosphoinositide-dependent kinase 1 ortholog; PDK-1 is a component of the DAF-2/insulin receptor-like signaling pathway and accordingly, functions to regulate such processes as dauer larvae formation, longevity, and salt chemotaxis learning; genetic analyses indicate that, in regulating dauer arrest, PDK-1 acts downstream of AGE-1/PI3K and upstream of the AKT-1 and AKT-2 kinases; a PDK-1::GFP fusion protein is expressed broadly beginning in late stage embryos and continuing on through adulthood; expression is seen in head, tail, and ventral cord motor neurons, pharyngeal tissues, hypodermal cells, the intestine, and the somatic gonad; in neurons, the PDK-1::GFP localizes to cell bodies and processes, with occasional expression seen in some neuronal nuclei. [ HYPERLINK /db/gene/gene?name=WBGendetails=1 \t _blank details] NCBI KOGs HYPERLINK /db/misc/glossary?name=KOGs;options=terms \t _blank *: [ HYPERLINK /db/gene/homology_group?name=OMpre_WH001712;class=Homology_group OMpre_WH001712]; Serine/threonine protein kinase [ HYPERLINK /db/gene/homology_gr