电化学发光法测定食品中四环素总量【毕业设计】.doc

- PAGE I - （20 届） 本科毕业设计 电化学发光法测定食品中四环素总量 The Total Amount of Tetracyclines in Food Determined by an Electrochemiluminescence Method 所在学院 专业班级 应用化学 学生姓名 学号 指导教师 职称 完成日期 年 月 电化学发光法测定食品中四环素总量 PAGE II PAGE III 电化学发光法测定食品中四环素总量 【摘要】目的：本文建立一个高灵敏度测定食品中四环素总量的电化学发光法，以蜂蜜作为样品进行测定。方法：基于四环素、土霉素、金霉素、强力霉素对三联吡啶钌的电化学发光具有几乎完全相同的增强效应，建立本方法，并阐明其机理。结果：在最优的实验条件下，体系中三联吡啶钌的电化学发光强度的增加值与四环素、土霉素、金霉素和强力霉素浓度的对数值具有线性关系，线性范围为1.0×10?12 ～5.0×10?10 mol · L?1，检测限为3×10?13 mol · L?1。结果精确度和准确度好。机理研究表明，是四环素的电化学氧化产物增强了三联吡啶钌的电化学发光强度。结论：该方法简单，灵敏度高，实验结果可靠，可用于食品中四环素总量的测定。 【关键词】四环素；三联吡啶钌；电化学发光；蜂蜜；总量。 The total amount of tetracyclines in food determined by an electrochemiluminescence method Abstract 【Abstract】Objective: To establish an electrochemiluminescence method with high sensitivity to determine the total amount of tetracyclines in food.The honey was determined as sample.Method: The ECL of Ru(bpy)32+ could be enhanced by tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC) identically.Base on it,this method was developed.And the enhancement mechanism was studied.Results:Under the optimized conditions,the enhanced ECL intensity of the system versus the logarithm of the concentration of TCs is linear over a concentration range1.0×10?12 ~5.0×10?10 mol · L?1,and the detection limit is 3×10?13 mol · L?1.The precision and accuracy are good.The mechanistic studies demonstrate that the electro-oxidation production of TCs enhance the ECL intensity of Ru(bpy)32+ .Conclusion: The proposed method is simple, with high sensitivity.And the results are reliable,then could be used for the determination of the total amount of TCs in food. 【Key words】tetracyclines; Ru(bpy)32+; electrochemiluminescence; honey; total amount. 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc292545862 1 引言 PAGEREF _Toc292545862 \h 1 HYPERLINK \l _Toc292545863 2 实验部分 PAGEREF _Toc292545863