农产品中几种有害染色剂检测技术的研究【毕业设计】.docVIP

- PAGE I - （20 届） 本科毕业设计 农产品中几种有害染色剂检测技术的研究 The detection of Several harmful dyes in agricultural products 所在学院 专业班级 化学 学生姓名 学号 指导教师 职称 完成日期 年 月 农产品中几种有害染色剂检测技术的研究 PAGE 20 PAGE I 摘　要 【摘要】建立了农产品中亮蓝和日落黄超高效液相色谱仪检测的检测方法。样品经乙醇+水+氨水（2/1/7，V/V/V）振荡提取，再经SAX固相萃取小柱净化，超高效液相色谱仪-紫外检测器检测。该分析方法对农产品中亮蓝添加回收率在84.9~99.0％之间，批内变异系数在2.9％～8.3％之间，批间变异系数为2.2~4.3%，最低检出浓度为0.1mg/kg。对日落黄添加回收率在84.9~99.5％之间，批内变异系数在3.5％～7.5％之间，批间变异系数为1.5~2.7%，最低检出浓度为0.1mg/kg。该法操作简便、灵敏、准确，适用于农产品中亮蓝、日落黄的测定。 【关键词】亮蓝;日落黄；胭脂红； 超高效液相色谱仪； SAX固相萃取小柱。 The detection of Several harmful dyes in agricultural products Abstract 【ABSTRACT】An analytical method was developed to determine residue of brilliant blue and carmine in agricultural products. Samples were extracted by alcohol+water+ammonia water(2/1/7,V/V/V), and further cleaned-up by SAX solid Phase extraction cartridge, and determined by UPLC-PDA. The average fortified recovery of brilliant blue in agricultural products was in the range of 91.7~99.0%, the interassay coefficient of variation was in the range of 2.9~8.3％, the intraassay coefficient of variation was in the range of 2.9~8.3％, the limit of detection was 0.1mg/kg.The average fortified recovery of Carmine in agricultural products was in the range of 84.9~99.5%, the interassay coefficient of variation was in the range of 3.5~7.5％, the intraassay coefficient of variation was in the range of 1.5~2.7％, the limit of detection was 0.1mg/kg.The method is simple, sensit 【KEYWORDS】brilliant blue; Sunset yellow; Carmine; UPLC-PDA; SAX solid Phase extraction cartridge。 目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc294159039 摘　要 PAGEREF _Toc294159039 \h II HYPERLINK \l _Toc294159040 Abstract PAGEREF _Toc294159040 \h III HYPERLINK \l _Toc294159041 1 引言 PAGEREF _Toc294159041 \h 1 HYPERLINK \l _Toc294159042 1.1亮蓝的相关检测技术 PAGEREF _Toc294159042 \h 2 HYPERLINK \l _Toc294159043 1.2日落黄的相关检测技术 PAGERE