HPLCELSD测定地黄寡糖中地黄苷A含量.pdfVIP

HPLC-ELSD 测定地黄寡糖中地黄苷 A 含量  刘影a a* b a ，杨菁 ，王晶 ，刘涛 (辽宁医学院，a.药学院；b. 附属第一医院，辽宁 锦州 121000) 摘要：目的 建立测定地黄寡糖中地黄苷A 的高效液相色谱法。方法 采用Agilent TC C18 色谱柱(4.6 mm×250 mm ，5 μm) ， 1 ；ELSD 参数：漂移管温度 110 ℃,载气流量3.2 L·min1。结果 柱温35 ℃，流动相：甲醇-水(10 ∶90) ，流速1.0 mL·min 地黄苷A 在0.2~1.2 mg·mL1(r=0.999 3) 内线性关系良好，平均回收率为98.8%(RSD=1.6%) 。结论 该方法准确、简便、 专属性好，可用于地黄寡糖中地黄苷A 成分的含量测定，为地黄寡糖的质量控制提供了依据。 关键词：地黄寡糖；地黄苷A ；高效液相色谱法；蒸发光散射检测器 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2013)11-1223-03 Determination of Rehmannioside A in Rehmannia Glutinosa Oligosaccharide by HPLC-ELSD a a* b a LIU Ying , YANG Jing , WANG Jing , LIU Tao (Liaoning Medical University, a.Faculty of Pharmacy; b.First Affiliated Hospital, Jinzhou 121000, China) ABSTRACT: OBJECTIVE To establish an HPLC method to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide. METHODS The HPLC-ELSD was used with Agilent TC C18 column (4.6 mm×250 mm, 5 μm) at 35 ℃. The mobile phase was methanol and water(10 ∶90) with a flow rate 1.0 mL·min1; The temperature of the drift tube in ELSD was 110 ℃ and the flow rate of dry air was 3.2 L·min1. RESULTS The rehmannioside A had a good linear relationship between 0.2-1.2 mg·mL1. CONCLUSION The method is accurate, simple with good specificity ，and it could be used to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide, which may be providing a evidence for the quality control of Rehmannia glutinosa oligosaccharide. KEY WORDS: Rehmannia glutinosa oligosaccharide; rehmannioside A; HPLC; ELSD 生地黄为玄参科植物地黄Rehmannia glutinosa 还具有滋阴的效果，增强体液免疫和细胞免疫功 Libosch 的干燥块根。具有清热凉血、养阴生津 能[8] 。 的功效，味甘，性寒，归心、肝、肾经[1] 。地黄 本实验