水果表面高产7一氨基丁酸酵母茵菌株的筛选鉴定和-浙江农业科学.PDF

浙江农业学报Acta 20(6)：396～401，2008 agriculturaeZhejiangensis 水果表面高产7一氨基丁酸酵母茵菌株的筛选、鉴定和 发酵条件优化 蒋冬花，李 杰，后家衡，徐晓波 (浙江师范人学化学与生命科学学院，浙江金华321004) 摘要：从水果表而分离筛选到1株相对高产弘氨基丁酸(GABA)的酵母菌菌株MJ2，经初步鉴定该菌株为啤 酒酵母(SaccharomycescerevLsiae)。对菌株MJ2的发酵培养基和发酵条件用单冈素轮换试验和正交试验进行 整体优化。结果表明菌株MJ2转化富集GABA的适宜条件为：以町溶性淀粉为碳源、牛肉膏为氮源，初始pH 5．5、培养温度3l℃、摇床速度180r／min时，发酵培养3 d，发酵液中GABA的产量最高可达7．539g／L。 关键词：y-氨基丁酸(GABA)；酵母菌；筛选；正交实验 中图分类号：Q935 文献标识码：A ofa acid fromthefruitsurfaceand ScreeninghighyieldY-aminobutyricyeast opti- mizationofitsculturalconditions JIANG Xiao-bo Dong-hua，LIjie，HOUJia—heng，XU Normal (CollegeofChemistryand职Science，ZkjiangUniversity，Jinhua，Zhejiang321004，China) of the Abstract：Astrain acid(GABA)wasisolatedfrom fruitsurface．Theselected yeastpreducdy-mninobutyrie yeast and (MJ2)was cerevisiae tothe and culturesubstrate identified器Saccharomycesaccordingmorphologybiochemistry．The conditionsof were f∽tor fermentation MJ2 and test．Theresultsshowed optimizedbysingle experimentt9(34)orthogonal the conditionsofGABA from starchas as that the f10luble carbon∞urce，bem,f optimum produced yeast(踟2)were paste 5．5atthe ofthe culture r／rainof culture，31℃of nitrogensource，pH beginning temperature，180shakingspeed．The contentofGABAw鹤7．539inthe cultureconditions． g／L optimum Keywords