甘蔗S-腺苷甲硫氨酸合成酶基因ScSAM的克隆及表达-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2014, 40(6): 1002−1010 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.01002 甘蔗S-腺苷甲硫氨酸合成酶基因(ScSAM) 的克隆及表达 宋修鹏1 张保青2 黄 杏2 杨丽涛1,2,* 李杨瑞1,2,* 1 / , 530004; 2 / / / , 530007 : RT-PCR RACE 22 (ROC 22) SAM cDNA , ScSAM, GenBank KC172558 , cDNA 1466 bp, 1 1191 bp (ORF), 396 , SAM , ScSAM SAM Real-time PCR ScSAM , , 3.6 (4 )(PEG)NaCl , ; H2O2 , : ; S-; ; Cloning and Expression of Sugarcane S-adenosylmethionine Synthetase Gene ScSAM 1 2 2 1,2,* 1,2,* SONG Xiu-Peng , ZHANG Bao-Qing , HUANG Xing , YANG Li-Tao , and LI Yang-Rui 1 Agricultural College / State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China; 2 Guangxi Academy of Agricultural Sciences / Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture / Guangxi Key Laboratory of Sugarcane Genetic Improve- ment, Nanning 530007, China Abstract: The full-length sequence cDNA of ScSAM (GenBank accession number: KC172558) was cloned from sugarcane vari- ety ROC 22 using RT-PCR combined with RACE techniques. This sequence consists of 1466