褪黑素对小鼠淋巴细胞电离辐射损伤的防护作用-中国辐射卫生.PDF

DOI :10.13491/j.cnki .issn.1004 -714x.2005.02.009 ·96 · 2005 6 14 2 　Chin J Radiol Health, June 2005, Vol 14, No 2 【】 ＊ 　,,　,　, ,　, :R818.7　:A　:1004-714X(2005)02-0096-04 　　【】　　(MLT)。　 DNA 。, 3 MLT, 2 Gy X 24 h 、H-TdR Con A、LPS T、B 。　, 0.5～6.0 Gy , 、DNA , 2 mmol/LMLT, 、DNA 。, 2Gy MLT, DNA , , MLT 0.1 ～2.5 m /k , 。 3 、、 H-TdR T、B 2 Gy (P0.001)。0.5～10 m /k MLT , 、0 m /k ( 3 ), 0.5 m /k ;H-TdR (P0.01P0.001), 0.5 m /k ;Con A T , 0.5 m /k ;LPS B , 10m /k 。　MLT ,。 【】　;;;; Protective Effects of Melatonin on Dama e of Lymphocytes in Mice Induced by Ionizin Radiation.ZHANG Xuan, WANG Zhen- qi, LIU Yan , et al.MH Radiobiology Research Unit, School of Public Health, Changchun 130021, China . 【ABSTRACT】　Objective 　To explore the protective effects of melatonin (MLT)on the dama e of lymphocytes in mice in- duced by ionizin radiation and its mechanism.Methods　The percenta es of apoptotic bodies and the DNA lytic rates of thymocytes and splenocytes in mice in vitro and in vivo were detected with flow cytometry and fluorospectrophotometry, respectively.MLT was iven to mice throu h intraperitoneal injection 60 min before the whole-body irradiation with 2 Gy X-rays.Twenty four hours after irra- 3 diation, the number of thymocytes and splenocytes, H-TdR incorporative rate (HTIR)of thymocytes and T and B lymphocyte trans- formin rates (TLTR and BLTR)induced by Con A and LPS were observed.Results　The apoptosis of mouse thymocytes and spleno- cytes in vitro increased