新型喹啉类Zn2+探针的合成与细胞成像研究.docVIP

新型喹啉类Zn2+探针的合成及细胞成像研究 匡红波1，王忠龙1，张燕1，徐徐1.2，谷文1.2，王石发1.2 * (1. 南京林业大学 化学工程学院，江苏 南京 210037；2. 江苏省生物质能源与材料重点实验室，江苏 南京 210037) 摘要：以2-甲酰基-8-羟基喹啉为原料，与苯肼进行缩合反应合成了荧光分子探针HSZn，采用FTIR、NMR、HRMS对其结构进行表征。紫外可见光谱和荧光光谱分析研究表明，该探针在水相中对Zn2+具有较好的荧光增强效应，而其他金属离子对其干扰较小；HSZn的荧光强度与Zn2+浓度(1.0×10-5~1.0×10-4 mol/L)具有良好的线性关系，检测限低至4.0×10-8 mol/L；Job’s曲线和LC-MS分析表明，Zn2+与HSZn的络合比为1:2，且探针在pH=5~13内对Zn2+都有较灵敏的荧光响应。细胞成像实验表明，该探针可在Hela活细胞中实现对Zn2+的荧光成像。 关键词: 8-羟基喹啉；希夫碱；锌离子；荧光探针；细胞成像 中图分类号：O622.6 文献标识码：A Novel Quinoline-Based Fluorescent Probes for Detecting Zn2+ in Living Cells KUANG Hong-bo1, WANG Zhong-long1, ZHANG Yan1, XU Xu1.2, GU Wen1.2, WANG Shi-fa1.2 * (1 College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China; 2 Key Lab of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, Jiangsu, China) Abstract: A Schiff-based fluorescent molecular probe (HSZn) was synthesized by condensation of quinoline with phenylhydrazine and characterized by FTIR, NMR and HRMS. The UV-Vis spectroscopy and fluorescence spectroscopy studies demonstrated that the probe showed a significant fluorescence enhancement effect toward Zn2+ in aqueous medium while suffering from a slight interference at the presence of other metal ions. A good linear relationship between the Zn2+ concentrations (1.0×10-5~1.0×10-4 mol/L) and the fluorescence intensity was obtained for determining the content of Zn2+ with a detection limit of 4.0×10-8 mol/L. A 1:2 binding ratio of Zn2+/HSZn for the complex was obtained by Job’s plot and LC-MS, HSZn also exhibited a sensitive fluorescence response?toward Zn2+ in the wide pH range (pH=5~13). Cell imaging studies indicated that the probe was capable of detecting endogenous Zn2+ in live Hela cells. Key words: 8-hydroxyquinoline; Schiff-base; zinc ion; fluorescence probe; cell imaging Foundation items: Supported by the Natural National Science Foundation of China (No; the Major Project of Jiangsu Province University Natural Science (No.14KJ22000