血管发生中的内皮祖细胞.pdfVIP

Acta Physiologica Sinica , February 25, 2005, 57 (1): 1-6 1 Brief Review Endothelial progenitor cells in angiogenesis XU Qing-Bo* Department of Cardiac and Vascular Sciences, St George’s Hospital Medical School, London, W17 0RE, UK Abstract: Circulating blood contains a subtype of progenitor cells that have the capacity to differentiate into mature endothelial cells in vitro and in vivo. These cells have been termed endothelial progenitor cells (EPCs). The isolation of EPCs by adherence culture or magnetic microbeads has been described. EPCs are characterized by the expression of 3 markers, CD133, CD34, and the vascular endothelial growth factor receptor-2. After differentiation, EPCs express CD31, vascular endothelial cadherin, and von Willebrand factor. Evidence is accumulating that EPCs can facilitate endothelial repair and angiogenesis in vivo. We observed that EPCs can regenerate damaged endothelial cells in vascular grafts in apoE-deficient mice, and that abundant vascular progenitor cells are present in the adventitia of the vessel wall. It is not clear yet, however, whether these EPCs are essential for these angiogenic and atherogenic processes. Moreover, there are still many uncertainties about how cardiovascular risk factors alter EPC function. Thus, further studies on the mechanisms of EPC homing, releasing and attaching will be of help to explore areas of potential basic research and clinical application of EPCs. Key words: EPC; bone marrow; stem cells; angiogenesis and atherosclerosis 血管再生中的内皮祖细胞 徐清波* 圣· 乔治医院医学院心血管科学系，伦敦，英国 摘 要: 循环血液里存在一种被称为内皮祖细胞(endothelial progenitor cells, EPCs)的祖细胞亚群，具有在体内外分化为成熟内皮 细胞的能力。根据内皮祖细胞与其他血液细胞的粘附能力的差异和内皮祖细胞的抗原特异性，内皮祖细胞可通过贴壁培养和 免疫磁珠筛选而分离获得。内皮祖细胞可特异性表达三种祖细胞分子标志：CD133 、CD34 和血管内皮生长因子受体-2 。当 内皮祖细胞分化为成熟内皮细胞后，血小板内皮细胞粘附分子-1 (CD31) 、血管内皮粘附素(VE-cadherin ，又称CD144)和Ⅷ 因子(vWF)表达将上调。越来越多的证据显示，内皮祖细胞有利于体内内皮损伤后修复和血管再生。我们的研究发现，内 皮祖细胞可修复apoE- 缺陷小鼠血管移植物中的损伤内