巴金森氏症病患血浆之蛋白质体研究Proteomicanalysisofthe.PPTVIP

Dissect the plasma protein markers for Parkinson’s disease Wang Vin-Chi, Lin Ching-Yu, and Chen Han-Min About Parkinson’s disease 1. History A neuron system disease (James Parkinson, 1817) The second major neurodegenerative diseases in the world (after Alzheimer disease, AD) 2. Direct cause The impairment of motor neuron cell in substantia nigra of midbrain. 3. Major Symptoms Generally, Parkinsons’ patients gradually lost the ability of writing, walking or showing facial countenance. Four clinical criteria tremor rigidity bradykinesia postural instability 4. Treatment From 1960, it is known that the Levodopa is effective to reduce symptom of 75% PD patients 5. The social cost on PD 2,500 USD / year /patient 56 hundred million / year / united state To identify specific protein marker(s) from the blood of patients with Parkinson’s disease. To develop a quick and convenient diagnosis method for Parkinson’s disease. Find in early days, treat in early Days. * Who is suffered from Parkinson’s disease? About Parkinson’s disease The neuronal death cause. Normal image Those neuron cells produce dopamine as neuro-transmitter. A. Death of motor neuron cells B. no dopamine C. body motion dis-coordinate. In the brain About Parkinson’s disease About Parkinson’s disease Diagnosis method Base solely on doctor’s experience by evaluating the suspected patients for the four mentioned symptoms. Disadvantages: Not 100% accurate Time consuming Labor consuming Can not be performed routinely Goal To identify protein markers in blood for PD by proteomic approach Approach Storage site for Genetic code Transporter for Genetic code Executor Why proteins? Protein: the true physiological executors Clinical diagnosis for blood samples What’s “proteomics” ? The analysis of the entire protein complement expressed by a genome, or by a cell or tissue type.“ Two MOST applied techniques in proteomics: 1. 2-D electro