加味七方胃痛颗粒对胃癌细胞失巢凋亡的干预研究-中医内科学专业论文.docxVIP

研究生优秀毕业论文 导细胞凋亡。机制可能为通过上调Fas／FasL系统通路相关因子的表达水 导细胞凋亡。机制可能为通过上调Fas／FasL系统通路相关因子的表达水 平，从而参与细胞的凋亡过程。 关键词： 七方胃痛颗粒，裸鼠原位移植，细胞凋亡，胃癌，Fas， FasL，FADD，Caspase一8。 万方数据 Effect Effect of modified Qifangweitong granule on gastric cancer cells apoptosis：an intervention study ABSTRACT Objective：This experiment by means of the Qifangweitong granule granule intervention in human gastric cancer cells SGC-790 1 transplanted into nude mice to detect Fas，Fasl，FADD，Caspase8 factor expressed in tumor,in order to study the Qifangweitong granule act on human gastric cancer nude mouse orthotopictransplantation cancer cell proliferation，discusses the Qifangweit- ong granule on the regulation of gastric cancer cell apoptosis effect，provide objective basis for TCM prevention and treatment of gastric cancer． Methods：(1)Repeating vaccinations represented in BalB／subcutaneous(nu ／nu)nude mice With gastric cancer cell line SGC．790 1．To be The formati． on of subcutaneous transplantation tumor and block material．And the block material would be used to Inoculate in the serosa layer of nude mice stomach After all，Observe the survival state and the growth of the CanCer．Building people gastric cancer cell line SGC一790 1 by nude mouse orthotopic transp． 1antation animal model．(2)By the tumor grow to 5mm，The mouses are divi． ded into four groups Ran—domly．And intervention group of nude mouse transplantation in situ carcino-ma by injecting lavage，Observed the state of survival and the growth of cane—er every day．(3)All nude mice were cervical dislocation executed in 2W，weighing the weight and calculating the inhi． bitory rate of tumor after complete stripping tumors．tumor inhibition rate 2(tumor weight of the control group experim—ental experimental group tumor weight)／tumor weight of the control group X 1 00％．(4)Detecting the expres． sion of the four groups in Fas，FasL，FADD，Caspase一8 gene in mRNA level 万方数据 were were determined by RT—PCR、Western blot and ELISA assay． Results：(1)The SGC-790 1 in nude mice