几丁质酶高产菌株的筛选及酶法制备几丁寡糖的研究-农产品加工及贮藏工程专业论文.docxVIP

IsoIation IsoIation of Chitinase—Producing Strains and Enzylnatic Preparation of Chitinoligosaccharides Abst阳cl A strain B22 producing chitinase was isolated矗om so订samples collected from Hain觚IslaIld，and was idemined as，印，07Hyc酷c已f胁地∥口v“5．The optimized medium for strain B22 to produce chitinase was as follows(g／L)：peptone 2，yeast extract 2，K2HP04 O．7，KH2P04 0．3，MgS04．7H20 O．5，FeS04．7H20 O．019，chitin powder 129，pH 6．8．ARer inoculated with 3％of starter in a 250mL nask containing 70mL medium，and incubated 96 h at 27℃aIld 180“min，the chitinase activity reached 2585 U／mL The chitinase of the stra