水稻抗白叶枯病近等基因系CBB30的培育与 Xa30(t)的初步定位.docVIP

水稻抗白叶枯病近等基因系CBB30的培育及 Xa30(t)的初步定位 金旭炜1，2，王春连2,3，杨 清1，江祺祥4，樊颖伦2,3，刘古春4，赵开军2,3 （1南京农业大学生命科学学院，南京 210095；2中国农业科学院作物科学研究所/农业部作物遗传育种重点实验室，北京 100081； 3国家农作物基因资源与基因改良重大科学工程，北京 100081；4江苏省常州市武进区稻麦育种场，常州 213149） 摘要：【目的】将普通野生稻资源Y238所携有的抗白叶枯病新基因Xa30(t)导入栽培稻JG30，培育近等基因系，进行分子标记定位，以便应用于育种实践。【方法】以感病籼稻品种JG30为轮回亲本，Y238为供体进行杂交、回交、自交培育近等基因系；以JG30/Y238的一个BC6F2代群体为定位群体，利用分离集团分析法（BSA），借助SSR、EST、STS等分子标记对Xa30(t)进行分子标记定位。【结果】成功培育了携有Xa30(t)基因的水稻抗白叶枯病近等基因系CBB30。从343个分子标记中筛选出4个能揭示抗感多态性的标记RM1341、V88、C189及03STS，用该4个标记对BC6F2代群体303个单株进行分子检测和连锁分析，结果表明上述4个标记均位于水稻第11染色体长臂，与Xa30(t)的遗传距离分别为11.4 cM、11.4 cM、4.4 cM及2.0 cM，且它们位于Xa30 关键词：水稻白叶枯病；Xa30(t)；近等基因系；分子标记定位 Breeding of Near-Isogenic Line CBB30 and Molecular Mapping of Xa30(t), a New Resistance Gene to Bacterial Blight in Rice JIN Xu-wei1, 2, WANG Chun-lian2, 3, YANG Qing1, JIANG Qi-xiang4, FAN Ying-lun2, 3, LIU Gu-chun4, ZHAO Kai-jun2, 3 (1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095; 2Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 3The National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081; 4Wujing Rice and Wheat Breeding Station, Jiangsu, Changzhou 213149) Abstract: 【Objective】A new rice bacterial blight resistance germplasm (Y238) was identified from the wild rice species Oryza rufipogon and the resistance locus was transferred to the cultivated rice to breed near-isogenic line. Molecular mapping of the resistance gene was carried out to put it into practical use in the future. 【Method】The resistance gene Xa30(t) in Y238 has been introduced into JG30 by cross, backcrosses and selfing. A BC6F2 population was used for molecular mapping of Xa30(t), and Bulked Segregant Analysis (BSA) was adopted to survey SSR, EST and STS molecular markers. 【Result】Four markers, RM1341, V88, C189 an