褪黑素抑制大鼠急性肺损伤时核转录因子一-cB表达.PDF

·604· ·论著· 褪黑素抑制大鼠急性肺损伤时核转录因子一-cB的表达 张俊毅 丁春华 凌亦凌 董彩凤谭家庆 羡晓辉 【摘要】 目的 探讨褪黑素(MT)对大鼠急性肺损伤(ALI)时肺脏的保护作用及其可能机制。方法将 96只SD大鼠随机分为4组：对照组、脂多糖(LPS)组、地塞米松(DEX)和MT组，每组24只。采用气道内滴 注LPS制备ALI大鼠模型；DEX和MT干预采用腹腔注射。分别于给药后3、6和12h活杀各组大鼠取肺组 织标本，检测肺组织中髓过氧化物酶(MPO)和超氧化物歧化酶(SoD)活性及丙二醛(MDA)含量；用免疫组 化方法检测核转录因子-gB(NF—KB)在肺组织的表达。结果LPS组各时闻点SOD活性较对照组明显降低 用MT及DEX均能显著缓解上述变化(P0．05或P0．01)，上述各指标以6h变化最为显著，分别达到峰 值或谷底。结论MT对ALI时肺脏的保护作用可能与MT清除自由基及抑制NF—gB的激活有关。 【关键词】褪黑素； 自由基f核转录因子一KB effectofmelatoninon factor—KB Inrats the ofnuclear acute Inhibitory expression during lungInjury zHANG Chun-hua，LlNG Xiao-hui． Jun-yi’，DlNG Yi-ling，DONGCai—Yeng，TANJia-qing，xlAN of BasicMedicalScience，HebeiMedical *Departmentpathophysiology，丁妇Schoolof University， 050017，Hebei，China Shijiazhuang author：DING Corresponding Chun—hua(Ernail：dch54@sina．corn) To the effectofmelatonin(MT)ontissue [Abstract]Objectiveprotective lung during investigate acute ratsandits mechanism．Methods injury(ALI)inpossible Ninety—six lung Sprague-Dawley(SD)rats were dividedintofour randomly groups：control MTtreatment 24ratsineach modelofALlwasestablished (DEX)and group，with group．Rat byinstilling LPS DEXandMTwere ratsineach weresacrificed intratracheally，and injectedintraperitonea儿y．AUgroup at3，6and12hoursafterintratrachealinstillationofLPS，andtissue wereharvested． lung samples and (MDA) MyeIoperoxidase(MP0)activity，superoxidedismutase(SOD)activitymalondiaidehyde contentsin tissue wered