湖北黄连叶斑病病原鉴定及其生物学特性-植物保护学报.PDF

植物保护学报Journal of Plant Protection ，2018 ，45 （2 ）：315-321 DOI ：10.13802/ki.zwbhxb.2018.2016154 湖北省黄连叶斑病病原鉴定及其生物学特性 席中刚 游景茂 郑 露 刘 浩 黄俊斌* （华中农业大学植物科技学院，湖北省作物病害检测和安全控制重点实验室，武汉430070 ） 摘要：为明确湖北省黄连叶斑病病原菌种类及其生物学特性，采用常规组织分离法获得病原菌菌 株HL- 1，通过形态学特征并结合基于ITS 序列分析对菌株进行鉴定，并研究该病原菌的生物学特 性，用生长速率法测定了6 种杀菌剂对该病原菌菌丝生长的抑制作用。结果表明，黄连叶斑病病原 菌分生孢子器黑褐色，大小为100.5~ 120.0 μm× 175.3~210.0 μm ；分生孢子无色，单孢，大小为2.0~ 2.5 μm×4.1~5.4 μm ；该菌株ITS 序列系统发育进化分析结果表明，其与耧斗菜茎点霉Phoma aquile- giicola （HM222537.1）的同源性为100%，通过形态学特征及分子鉴定结果确定该菌株为耧斗菜茎 点霉。该病原菌在4~25℃内均能生长，最适温度为20℃；不同光照条件对病原菌生长的影响无显 著差异；在供试碳（氮）源中病原菌对麦芽糖和蛋白胨的利用效果最好；菌丝的致死温度为 50℃、10 min 。供试6 种药剂中10%苯醚甲环唑对病原菌菌丝生长的抑制效果最好，其EC50 最小， 为0.03 μg/mL 。 关键词：黄连；叶斑病；病原菌；生物学特性 Identification and biological characteristics of the pathogen causing leaf spot on Coptis chinensis in Hubei Province Xi Zhonggang You Jingmao Zheng Lu Liu Hao Huang Junbin* （The Key Laboratory of Plant Pathology in Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China） Abstract: In order to confirm the pathogen of leaf spot on Coptis chinensis and its biological character- istics, the strain HL- 1 was isolated by tissue isolation method; the pathogen was identified with morpho- logical characteristics and ITS sequence analysis, and the biological characteristics were also deter- mined. The toxicities of six fungicides against the pathogen were tested. The results showed that the fun- gal pycnidia were black brown, 100.5-120.0 μm× 175.3-210.0 μm in diameter; its conidia were color- less and monospora, 2.0-2.5 μm×4.1-5.4 μm in diameter. The analysis of ITS rDNA sequence showed that the fungus shared 100%identity with Phoma aquilegiicola (HM222537.