滴定法与比色法检测银耳中亚硫酸盐含量.PDF

362 2009, Vol. 30, No. 24 食品科学 ※分析检测 魏丽红 (辽宁农业职业技术学院农产品质量安全检测中心，辽宁营口 115009) 采用蒸馏法和浸泡法提取银耳中亚硫酸盐，滴定法与比色法测定亚硫酸盐含量。结果表明：在蒸馏提取 法和浸泡提取法中，滴定法和比色法相对标准偏差分别在1.364%～4.719% 和1.037%～4.480% 。两者可以作为检 测银耳中亚硫酸盐的方法。提取液中亚硫酸盐含量的测定首选滴定法，此法简单、快速，回收率在79.41% ～ 82.11%，相对标准偏差(RSD)为0.231%～0.620% ，蒸馏滴定法与浸泡滴定法检出限分别为0.010g/kg 和0.015g/kg ， 结果可靠；比色法结果偏低，回收率79.08%～80.80%，RSD 为0.609%～0.746% ，蒸馏比色法和浸泡比色法的检 -3 -3 出限分别为1.2 ×10 g/kg 和1.5 ×10 g/kg ，适合于大批样品同时测定。 银耳；亚硫酸盐；比色法；滴定法 Use of Titration and Spectrophotometry for Determination of Sulfite in Tremella WEI Li-hong (Agricultural Product Quality and Security Detecting Center, Liaoning Agricultural College, Yingkou 115009, China) Abstract ：Sulfites in tremella were extracted with NaOH solution or by distillation with water, respectively, and determined using titration or spectrophotometry methods. Both extraction methods and determination methods were compared. The RSD (relative standard deviation) of distillation and extraction with NaOH solution were 1.364% －4.719% and 1.037% －4.480% respecitvely, and both were applicable for the extraction of sulfite. The recovery of titration was 79.41%－82.11%, and RSD was 0.231% －0.620% with the detection limit at 0.010 g/kg and 0.015 g/kg. Compared with titration, colorimetry method was not fit for accurate determination, with the recovery 79.08% －80.80%, RSD 0.609%－0.746% and detection limit at 1.2 × -3 -3 10 g/kg and 1.5 ×10 g/kg, and suitable for batch samples as it was less time-consuming. Key words ：tremella ；sulfite ；colorimetry ；titration 中图分类号：TS202.3 文献标识码：A 文章编号：1002-6630(2009)24-0362-04 亚硫酸盐是一类很早即在世界范围内广泛使用的食