病毒引物设计.doc

PAGE PAGE 42 内封面 本科毕业设计（论文） 题目 H1N1流感病毒H1亚型PCR快速 检测方法的引物设计 院 ( 系 ) 专业名称 年级班级 学生姓名 指导教师 2011年 5 月 河南理工大学毕业设计论文 摘要 摘 要 目的：利用生物信息技术对H1N1流感病毒H1亚型设计PCR快速检测方法的引物。方法：利用DNAStar的MegAlign软件对H1N1流感病毒与从NCBI Genbank下载的其他亚型流感病毒核酸序列进行比对，并对H1亚型不同毒株的HA序列之间进行核酸序列比对。最后利用primer premier5.0设计引物，并对设计的引物在NCBI中进行BLAST。结果：通过MegAlign软件比对发现，H1亚型流感病毒HA序列与其他亚型的HA序列核酸序列同源性较差，而H1亚型不同毒株的HA序列较为保守，通过筛选设计出了较好的引物，使H1N1流感病毒H1亚型PCR的检测更加迅速、准确。结论：H1N1流感病毒H1亚型与其他亚型的核酸序列同源性较差，而H1亚型不同毒株的HA序列较为保守，应用软件设计的H1亚型的引物可以较好地检测出H1亚型流感病毒。 关键词：H1N1流感病毒 H1亚型 快速检测 PCR引物设计 河南理工大学毕业设计论文 ABSTRACT ABSTRACT Objective: To design H1N1 influenza virus H1 subtype PCR primers for rapid detection method with biological information technology. Methods: Compare nucleic acid sequences from H1N1 influenza virus and other subtypes of flu virus download from Genbank NCBI with MegAlign of DNAStar software and HA nucleic acid sequences from the different strains of H1 subtypes.Finally, design primers with the primer premier5.0, and Blast the primers in NCBI. Results: Through the MegAlign of DNAStar software ,we find that the homology of nucleic acids sequences between H1N1 influenza virus and other subtypes is poor, however, the nucleic acids sequences of different strains of H1 subtypes are more conservative. A pair of better primers were designed by screening from the results in primer premier5.0, making the PCR detection of H1N1 influenza virus more quickly and accurately. Conclusion: The homology of nucleic acids sequences between H1N1 influenza virus and other subtypes is poor, however, the nucleic acids sequences of different strains of H1 subtypes are more conservative. H1N1 influenza virus H1 subtype primer designe by software is better able to detect the H1 subtypes of flu viruses. Key words: H1N1 influenza virus H1 subtype rapid detection Design PCR primers 河南理工大学毕业设计论文