激光拉曼双光镊系统的研制和应用及单个大米淀粉微的糊化特性的实时分析-理论物理专业论文.docxVIP

PAGE PAGE IV Development and application of dual-trap Raman tweezers system and real-time study of the gelatinization process of a single rice starch granule Graduate student: Yan Li Supervisors: Prof. Jun-xian Liu, Prof. hui-luYao Specialty: Theoretical Physics Direction: Biophysics Entrance year: 2007 Abstract Optical manipulation and analysis of single particles in a native aqueous medium are extensively applied to biology and physics. Optical tweezers technology has become a hot topic of new method and new instrument in biophysics, because rapid, noninvasive analyses of individual particles held in an optical trap. Raman tweezers is combination of Raman spectroscopy with optical tweezers, it have many merits comparing to traditional microscopic Raman system: Raman tweezers utilize a focused laser to form the gradient force for trapping living cell in the optical trap. This design optimizes the collection optic of Raman scattered and obtains a high ratio of signal to noise. Stray light from the glass slides significantly decreased as a cell is captured about 10 microns upon the slide. So the combination of optical tweezers with optical spectroscopy allows analyses of individual particles held in an optical trap by identifying chemical composition and revealing molecular behavior of the individual particles suspended in complex environments directly. But a single-trap Raman tweezers can only track and analyze one particle or one cell at a given time. It cannot track or detect two or more individual cells and two correlated portions of one cell in the same time. This is a particular problem if we want to monitor dynamic behaviors of the two interacting cells (such as budding cells) in liquid suspension or monitor molecule transportation in different locations within one large cell. In this paper, we report on the first development of a dual-trap laser tweezers Raman spectroscopy system for the manipulation and analysis of multiple individual living cells. Dual-t