甜菜遗传连锁图谱初步构建-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2014, 40(2): 222−230 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.00222 甜菜遗传连锁图谱初步构建 王茂芊1,2,3 李 博1 王华忠1,2,3,* 1 / , 150080; 2 , 150080; 3 , 150080 : JV34-2 2B023 , 200 F2 , 56 SRAP 20 SSR , F2 PCR , 9 141 (123 SRAP 18 SSR) 1399.88 cM, 9.92 cM4 9 3~26 , 15.69~237.21 cM 20.56%, Chr.3 , Chr.1Chr.2Chr.8 Chr.9 SRAP SSR , , : ; SRAP; SSR; Construction of Molecular Genetic Linkage Map of Sugarbeet 1,2,3 1 1,2,3,* WANG Mao-Qian , LI Bo , and WANG Hua-Zhong 1 Key Laboratory of Sugar Beet Genetic Breeding/ Crop Academy of Heilongjiang University, Harbin 150080, China; 2 Sugar Beet Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150080, China; 3 Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Acad- emy of Agricultural Sciences, Harbin 150080, China Abstract: Molecular genetic map is a fundamental tool for genomic research. In this study, a molecular genetic map was con- structed using an F2 population consisting of 200 individuals from a cross between sugarbeet JV34-2 (the high beet yield and low sugar content line) and sugarbeet 2B023 (the low beet yield and high sugar content line) and a total of 561 SRAP primers and 114 SSR primers. Approximately 56 out of 561 and 20 out of 114 primers, respectively, showed polymorphisms. Each of these poly- morphic primers produced at least one scorable polymorp