大丽轮枝菌寡糖素诱导的棉花组培细胞的过氧化物酶-植物科学学报.PDF

2002, 20(6) : 457462 J ournal of Wuha n Botanica l Resear ch 刘曼西　荆迎军 ( , 430074) : 3 d (V44) ( V64) , (S) (R)( ) 4 d , , (H, V44 L, V64) , ( S R) (POD): ( 1) ; (2)6 ( ) ,( S ) ( S L(H) S) , 36 h POD , ( ) , ; (3) L H S (8 ) ( 12 ) R() R L ( ) 36 ( ) , 9 24 H R h POD L H R H R H S h h R H R R H S 6 h 9 h, : ; ; ; : Q945; S432. 1: A: 1000-470X(2002) 06-0457-06 The Peroxidase Isoenzymes of Suspending Culture Cells of Cotton Induced by the Oligosaccharins from Vert icillium dahliae LI Man-Xi, JING Ying-Jun ( Colleg e of L if e S cience and T echnology , H uaz hong Univ ersity of S cience and Tech nology, Wuhan430074, China) Abstract: The living cell cultureof cotton or its homogenates from V .dahliae-susceptible variety (S) or resistant variety (R) were added into the Czapeks liquid media which had been inoculated 3 d with the higher virulent strain(V44) and lower virulent strain(V64) of V.d ahliae respective- ly. After 4 d additional incubation, the spore of these fungus were collected each to evaluate their wilting or virulenceto cotton plants and the cell wall of the hypha were used to prepare oligosac-, ( , 44 , 64) charins H from V and L from V which were as elicitors to induce the cell cultures of cot- .( ) ( 1) ton S or R T he PAGE spectrums of POD isozymes from cotton cells showed that all oligosaccharins induced cotton cells to produce new POD isozymes; ( 2)the living cotton cells or its homogenate from susceptible variety