内生真菌感染对同地理种群羽茅的生理生态影响（.docVIP

内生真菌感染对不同地理种群羽茅的生理生态影响( 贾彤 任安芝 尹立佳 魏茂英 高玉葆* （南开大学生命科学学院，天津，300071） 摘 要：Neotyphodium内生真菌与宿主的共生关系问题一直是一个研究的热点，而实际上二者的关系可能比认识到的更为复杂。本文为田间盆栽实验，研究了内生真菌对的羽茅在不同生长阶段的影响。研究结果表明，Neotyphodium内生真菌对羽茅的影响与宿主羽茅所处的原生生境和不同的生长发育阶段有关，羽茅生长前期，内生真菌显著提高了海拉尔种群植株的株高、比叶重、水分利用效率以及光合氮利用效率；羽茅生长后期，内生真菌对锡林浩特定位站羽茅的光合氮利用效率有显著的贡献。感染内生真菌对两个地理种群中羽茅的生物量的分配，叶绿素含量，叶片氮含量都没有显著的影响。 关键词：Neotyphodium内生真菌；羽茅；光合特性中图分类号： 文献标识码：201104-717 Ecophysiological effect of endophyte Infection on Achnatherum sibiricum in different populations in Inner Mongolia Steppe JIA Tong, REN Anzhi, YIN Lijia, WEI Maoying, GAO Yubao* (College of Life Sciences, Nankai University, Tianjin 300071) Abstract: It has been a focus of research about the relationship between Neotyphodium and host symbiosis, but in fact, the relationship between them may be more complicated than realized. In this experiment we studied the effect of endophyte infection in different growth stages of A. sibiricum between different populations in Inner Mongolia Steppe. The results showed that the effect of Neotyphodium on A. sibiricum was related to the original environment and different growth stages of A. sibiricum. At seedling stage, endophyte infection significantly increased plant height, leaf mass per area, water use efficiency and photosynthetic nitrogen use efficiency of Hailar population. At later growth stage, endophyte infection made a significant contribution to the photosynthetic nitrogen use efficiency of Xinlinhot population. There were no significant differences in biomass distribution, chlorophyll content and leaf nitrogen content of A. sibiricum in two populations. Key words：Neotyphodium; Achnatherum sibiricum; photosynthetic characteristics; original environment 0 引言 内生真菌的分布十分广泛，其宿主植物涉及藻类、针叶树、灌木和草本等多个类群，尤以禾本科植物最为常见。从世界范围来看，目前已至少在80个属290多种禾本科植物中发现有内生真菌[1]。广义的内生真菌指在其生活史一定阶段或全部阶段生活于健康植物各种组织和器官的细胞内部或间隙的真菌，包括生活史中某一阶段营表面生的腐生真菌，对宿主暂无伤害的潜伏性病原真菌和菌根真菌[2-4]。狭义指生活于健康植物的各种组织和器官的细胞内部或间隙的真菌[4] 。本研究中羽茅分离的内生真菌，以菌丝形式定居于宿主植物体内在自然情况下不产生子座和孢子只通过菌丝生长进入宿主植的繁殖器官经种子