丹酚酸B脂质体的制备及其体外释药的研究.pdfVIP

中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 1 期 2011 年 1 月 • 65 • 丹酚酸 B 脂质体的制备及其体外释药的研究 1, 2 1, 2 1, 2 马金鹏 ，舒乐新 ，刘志东 1. 天津中医药大学中医药研究院，天津 300193 2. 天津中医药大学 现代中药发现与制剂技术教育部工程研究中心，天津 300193 摘 要：目的 研究丹酚酸 B 脂质体的制备方法及其体外释放情况。方法 采用逆向蒸发法制备丹酚酸 B 脂质体，超滤离 心法测其包封率，以包封率和粒径为指标，采用正交试验设计法优化处方，并对其表面特征、包封率、粒径、体外释放情况 进行考察。结果 制备的丹酚酸B 脂质的体平均粒径为 109.8 nm，药物的平均包封率为71.0%，体外 12 h 累积释放率为43.7% 。 脂质体外观圆整而均匀，分散性好。结论 制备的丹酚酸 B 脂质体包封率较高，粒径小，有良好的缓释作用，为后期研究 其长循环心靶向脂质体奠定了基础。 关键词：丹酚酸B ；脂质体；体外释放；正交试验；分散性 中图分类号：R284.2; R286.02 文献标志码：A 文章编号：0253 - 2670(2011)01 - 0065 - 04 Preparation of salvianolic acid B liposome and its in vitro release MA Jin-peng1, 2，SHU Le-xin1, 2，LIU Zhi-dong1, 2 1. Research Center of Traditional Chinese Medicne, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2. Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Tradi- tional Chinese Medicine, Tianjin 300193, China Abstracts: Objective To study the preparation of salvianolic acid B liposome and its in vitro release. Methods Reverse-phase evaporation technique was chosen for preparation salvianolic acid B liposome and entrapment efficiency was measured by ultrafiltration. Orthogonal design was used to optimize the formulation,by taking encapsulation efficiency and the particle size as indexes. The properties of liposomes including morphology, entrapment efficiency, mean diameter, and accumulative release were studied. Results Mean diameter and encapsulation efficiency of prepared salvianolic acid B liposome were 109.8 nm and 71.0%, respectively. The in vitro 12 h accumulative release was, 43.7% of the total drug. The liposome was smooth and spherieal in appearance with better dispersion. Conclusion The salvianolic acid B liposome prepared