不同因素对花生总DNA提取的影响.pdfVIP

华北农学报 2009, 24 (增刊 ) : 16-19 DNA 罗秋婷, 钟 君, 满玉萍, 郭旭英, 严海燕 ( , 430074) : CTAB DNA DNA, 15, 1 0000 r/m in 30 m in 7 DNA, DNA, DNA . DNA, 15 DNA : DNA ; DNA; 15 : Q781 : A : 1000- 7091( 2009) - 0016- 04 Effects of D ifferent Factors on Quality of Genom ic DNA Extraction in Peanut UO Q iu-ting, ZHONG Jun, MAN Yu-ping, GUO Xu-ying, YAN H a-i yan ( College of ife Sciences, South-CentralUniversity forN ationalities, Wuhan 430074, China) Abstract: To explore the factors on CTAB m ethod of plantDNA extraction that affect the quantity and quality, seven methods phenol-chloroform, 10 000 r/m in, chloroform, no cutting on peptips, one step w ith chloroform, one stepw ith phenol, no shaking for 30 m in have been used to extract genom ic DNA from peanut leaves ofA rach is hy- p og aea. The DNA samples obtained by abovem ethodswere examed by appearance, agarose gel electrophoresis and ultraviolet spectrophotometry. Based on the comparative analysis of yield and quality of the DNA samplesby differ- ent methods, consideration all index of yield, protein content and the integrity of DNA molecules, the phenol-chloro- form method was found to be themost suitable for extraction of genom ic DNA from leaves ofA rach is hyp og aea. K ey words: DNA extraction; Genom ic DNA;A rach is hyp ogaea DNA , DNAPCR, DNA , Southern, DNA DNA , DNA DNA, 2 DNA : CTAB DNA , 10000 , DNA ase DNA ; r/m in [ 1, 2] DNA 30m in 7 DNA, , DNA , CTABDNA DNA, D