不同干馏时间对竹沥中化学成分的影响Δ本栏目协办.pdfVIP

·中药分析与鉴定· 不同干馏时间对竹沥中化学成分的影响Δ ＊ # 李 红 ，蒋孟良 ，金晓飞，尹 谦，杨 欣，吴治谚（湖南中医药大学，长沙 410208） 中图分类号 R284.1；R284.2 文献标志码 A 文章编号 1001-0408（2013）35-3305-04 DOI 10.6039/j.issn.1001-0408.2013.35.13 摘 要 目的：比较不同干馏时间下竹沥中化学成分的差异。方法：分别取干馏10min 与干馏50min 的样品，采用气相色谱-质谱 联用技术进行成分分析。色谱柱为DB-23毛细管（30m×0.25mm×0.25μm ），程序升温，进样口温度为250℃，载气为氦气，流速为 1ml/min ，分流比为5∶1，离子源温度为200 ℃，接口温度为250 ℃，质量扫描范围为35～800amu。结果：干馏10min 的竹沥检出 100个峰，可定性成分为38个，质量分数占全部检出峰的38％；干馏50min 的竹沥检出91个峰，可定性成分为39个，质量分数占全 部检出峰的43％。结论：干馏10min 与干馏50min 的竹沥成分差异较大，只有24个成分相同；且干馏50min 所得成分的平均相对 分子质量较干馏10min 大。 关键词 竹沥；炮制工艺；化学成分；气相色谱-质谱联用技术 Influence of Different Carbonization Time on Chemical Composition of Bamboo Juice LI Hong ，JIANG Meng-liang ，JIN Xiao-fei ，YIN Qian ，YANG Xin ，WU Zhi-yan （Hunan University of TCM ，Ch- angsha 410208，China ） ABSTRACT OBJECTIVE ：To study chemical composition of bamboo juice ，and to compare the difference of chemical compo- nents during different carbonization time. METHODS ：The compositions of bamboo juice after distilled for 10 min and 50 min were analyzed by GC-MS. The determination was performed on DB-23 capillary column （30 m× 0.25 mm× 0.25 μm ），by tempera- ture programming ，using helium as carrier gas at the flow rate of 1ml/min. The injector temperature was 250 ℃，and split ratio was 5∶1. The temperature of ion source was 200 ℃，and interface temperature was 250 ℃. The scanning range of ion mass was from 35 amu to 800 amu. RESULTS ：100peaks were detected after bamboo juice was distilled for 10min. 38 compounds were identified ，accounting for 38％ of the total peaks. 91 compounds were detected and 39 compounds were identified after bamboo juice was distilled for 50min ，accounting for 43％of the total peaks. CONCLUSIONS ：The composition of bamboo juice after dis- tilled for 10min and 50min have great difference ，and only 24 compositions are mutu