平菇培养料青绿霉菌的分离鉴定及特性.pdfVIP

广东农业科学 2013 年第4 期 57 平菇培养料青、绿霉菌的分离鉴定及特性 覃 升 刘 斌 黎金锋 黄福常 广西大学农学院应用微生物研究所袁 广西 南宁 530005冤 摘要 从南宁市平菇栽培原料尧污染菌包尧污染菌种和霉变子实体中分离青尧绿霉菌袁获得菌株47个袁采用形态学比较观察和 rDNA ITS1-5.8S-ITS2基因片段进行序列分析袁结果共鉴定出侧耳木霉尧绿色木霉尧哈茨木霉尧桔青霉尧 姆青霉和短密青霉6个种遥 6个菌株均在40益以上死亡袁湿度在40%以下不能生长袁pH值高抑制其生长遥 关键词 青尧绿霉菌曰ITS 曰生物学特性 中图分类号 S646.140.1 文献标识码 A 文章编号 1004-874X 2013冤04-0057-04 Isolation, identification and characteristics of Penicillium and Trichoderna from polluted oyster mushroom QIN Pei-sheng, LIU-Bin, LI J in-feng, HUANG Fu-chang (Institute of A pplied Microbiology, A gric ultural College , Guangxi Unive rs ity, Nanning 530005,China) Abstract: Forty-seven strains of Penicillium and Trichode rma were isolated from materials,substrate bags,spawn and fruiting body of oyster mushroom Pleurotus sp. from Nanning mushroom farms. They were identified to 6 species of Trichoderma pleuroticola, Hypocrea v irens , Hypoc rea lixii, Penic illium c itrnum Thom,Penic illium thomii and Pe nicillium brecicompactum according morphological characteristics and rDNA ITS1-5.8S-ITS2 sequences.In order to control these strains rightly, the text was also focused on the clarificatiom of their corresponding biological characteristics.Their growth optimum temperatur and pH was 25益 and 6 耀7,resperctively.They could produce conidia easily at high temperatures and urder dry conditions. Key words: Penicillium and Trichoderma; ITS; biological characteristics 平菇 Oyster mushroom冤狭义上是指侧耳属 Ple urotus冤 它们常寄生在平菇培养料和子实体上袁 由于其扩散蔓延 中的糙皮侧耳 Ple urotus ostreatus冤袁目前泛指侧耳属中主 [5-8]