绵羊prp基因型分析及组织特异性表达的研究-预防兽医学专业毕业论文.docxVIP

AbstractTransmissible Abstract Transmissible Spongifurm Encephalopathies(TSEs)are associated with pathological cellular prion protein，which called priori．The disease is slowly progressive fatal neurodegenerative disorders in humans and animals including variant Creutzfeldt-Jakob disease(vCJD)in humans，scrapie in sheep and goats，and bovine spongiform encephalopathy(BSE)in cattle，etc．Pfion is unique in physieal—chemical and biology properties compared with common pathogens，SO far nucleic has been found in prion The disease prevailed in many places in the world．The morbidity of TSE varies with different genotype of sheep The aggregation of PrP5。in the central system result in scrapie．The PrP gene expression WaS suggested in different organs of sheep using real—time RT-PCR． Meanwhile，the polymorphism was determined prion protein gene in sheep．The results laid solid foundation to further study the prion pathogenesis． 1 Susceptibility to natural scrapie in sheep is associated with polymorphisms at codons 136，154 and 171 of the prion protein rPrP)．To assess the risk of scrapie in sheep raised in China,DNA from 20 sheep of two breeds Was isolated，amplified and sequenced fur the PrP gene．The ovine PrP gene was found to be highly homologous The genotype associated with high susceptibility to scrapie(VRQ)was absent，whereas that associated with the resistance(ARR)Was present in 10％of sheep examined．ARK Was also rare(10％)．ARQ that is aSsociated with intermediate susceptibility was the genotype observed in the most ofsheep examined(80％)． 2 Herein we report meaSurement of sheep PrP mRNA using absolute quantitative real—time transcription and polymerase chain reaction(RT-PCR)．Total RNA Was isolated from different regions of the central system(CNS)of eighteen sheep and PrP mRNA WaS quantified by real-time RT-PCR using externally calibrated standard constructed with the recombinant PrP and B-actin plasmid．The results showed that high levels of PrP mRNA were expressed in all regi