清胃黄连丸快速TLC鉴别与小檗碱新荧光扫描定量.pdfVIP

·药物分析与检验· 清胃黄连丸快速TLC 鉴别与小檗碱新荧光扫描定量 封淑华，韩桂茹* ，赵志军(河北省药品检验所，石家庄 050011) 摘要：目的 建立清胃黄连丸的快速TLC 鉴别及小檗碱的高灵敏度荧光扫描定量方法。方法 采用同一供试品溶液在2 块薄层板上鉴别黄芩、牡丹皮、连翘、栀子和甘草，用荧光扫描法测定了清胃黄连丸中盐酸小檗碱的含量。结果 盐酸 小檗碱点样量在0.019 68~0.098 4 g 内呈良好的线性关系。盐酸小檗碱平均回收率为99.41% ，RSD 为1.34%(n=9) 。结论 TLC 鉴别斑点清晰，阴性无干扰。清胃黄连丸的快速TLC 鉴别与小檗碱新荧光扫描定量，简便、快捷、准确、实用，有 效提高了传统丸剂的质量检测速度。 关键词：清胃黄连丸；快速薄层色谱法鉴别；盐酸小檗碱；荧光扫描 中图分类号：R284.1 文献标志码：A 文章编号：1007-7693(2012)06-0524-06 Fast Identification by TLC and Quantitative Determination by Fluorescence TLCS for Qingwei Huanglian Pills * FENG Shuhua, HAN Guiru , ZHAO Zhijun(Hebei Provincial Institute for Drug control, Shijiazhuang 050011, China) ABSTRACT: OBJECTIVE To establish a method for fast identification by TLC and quantitative determination by fluorescence TLCS for Qingwei Huanglian pills. METHODS Scutellariae Radix, Moutan Coxtex, Forsythiae Fructus, Gardeniae Fructus, Glycyrrhizae Radix et Rhizoma were identified by TLC, using the same sample solution in two TLC plate. The content of berberine hydrochloride in Qingwei Huanglian pills was determined by fluorescence TLCS. RESULTS The methodological study showed a good linear correlation in the range from 0.019 68 to 0.098 4 μg of the berberine hydrochloride. The average recovery of berberine hydrochloride (n=9) was 99.41%, and RSD was 1.34%. CONCLUSION The method is simple, accurate, repeatable and with good precision. The method can be used for quality control of Qingwei Huanglian pills. The detection speed was effectively improved for assay of traditional pills. KEY WORDS: Qingwei Huanglian pills; fast identification by TLC; berberine hydrochloride; fluorescence TLCS 清胃黄连丸为中国药典历版收载的品种，由牡 1 仪器与试药 丹皮、连翘、黄连、栀子和甘草等 14 味中药组成， 日本岛津公司CS-930 和瑞士 CAMAG TLC 为大蜜丸和水丸2 种剂型。原标准项下[1] ，收载了 Scanner