黄芪对糖尿病大鼠心肌中心肌营养素1、血管紧张素Ⅱ表达的影响-内科学·内分泌与代谢病专业毕业论文.docxVIP

蔺州幺乎◎ 蔺州幺乎◎ 兰州大学硕士学位论文 乱，细胞核大小不甚规则，细胞浆相对淡染，心肌纤维排列紊乱、局灶性坏死， 黄芪干预组大鼠亦存在心肌细胞和心肌纤维的坏死，较糖尿病组有所减轻。3．糖 尿病组大鼠心肌组织中Ang．II明显高于正常组大鼠(尸0．01)，黄芪干预组较 模型组减少，差异有统计学意义(P0．05)，CT-lmRNA及蛋白的表达糖尿病 组高于正常组(P0．01)，黄芪干预组较模型组减少(尸o．01)。 结论：l、高糖高脂饲料喂养结合小剂量链脲佐菌素注射可成功复制糖尿病 大鼠模型，且在糖尿病成模后8周大鼠出现了心肌的病变；2、CT-lmRNA和蛋 白的及Ang—II在糖尿病组中的表达是升高的，可能参与了糖尿病心肌病变的发 生发展。黄芪注射液可以降低CT一1和Ang-II的水平，其具体机制需进一步研究。 总之，CT-1在糖尿病心肌病变的发生中起到一定作用，黄芪注射液可以减轻糖 尿病大鼠的心肌病变，减少CT-1的表达，同时减少心肌组织中Ang．II的表达。 关键词：糖尿病；心肌病变；心肌营养素．1；黄芪注射液；血管紧张素．II； 心脏指数； Ⅱ 萄州大乎⑩ 萄州大乎⑩ 兰州大学硕士学位论文 Influence of Radix Astragali inj ection on expression of cardiotrophin—·1 and angiotensin·-II in rats with diab etes ABSTRACT obiective：To understand the diabetic rat model of myocardial tissue of Cardiotrophin-I(CT-I)mRNA and protein expression，Discussion whether the cardiotrophin一1 is involved in the occurrence of diabetic cardiomyopathy．To investigate the mechanism for Astragalus to improve the diabetic rat cardiac lesions in rats． Methods：Male Wistar rats were randomly divided into normal group(n=1 0)、 diabetic model group(n 2 1 0)and astragalus group(n=l O)．The normal group were feeded、Ⅳitll normal forage plants(12％protein，5％fat，67％sugar，1 6％else)．Diabetic model group and astragalus group induced by tligh-fat diet(66．5％normal forage plants，20％sugar，2．5％cholesterin，l O％lard，1．0％bile salt)and STZ(30mg／kg)．Then astragalus group was given intragastric administration、析th astragalus at the dose of 89／kg／d for 8 weeks．Take the weight of rat heart,calculate cardiac index in rats， determination of myocardial fiber content in rats．Using mdioimmunoassay to measured the Ang-II content in myocardial tissue．111e mRNA expressions of CT-1 Was determined by RT-PCR,the protein expressions of CT-1 Was determined by immunohistochemistry． Results：1．Compared埘tll normal group，triglyceride，cholesterol，low-density lipoprotein were significantly higher in the diabetes group and the astragalus group 俨o．0 1)，high—density lipoprotein were lower in the diabetes group and the astragalu