棉花抗黄萎病及重要农艺性状QTLs定位研究-作物遗传育种专业毕业论文.docxVIP

AbstractTlle Abstract Tlle cotton venicillium wilt’wllich h勰become a maill obstacle fbr hi曲and stable yield in cottoIl，is called“me cancer ofcotton，，in Cllina．Thercfore it’s urgent to study the molecular ge=rIetics for cotton venicillium wilt．h1 present rcsearch，QTLs for anti—Vcnicilli啪win柚d岫portallt agro】10mic仃ai协werc m印ped by using SSR m01ecular make塔iIl F2 p叩ulaIioIls．The g∞etic ef‰ts fbr single locus werc aIso sttldied．This snldy will help to pmmote me development of molecular船sisted selcction i11 cotton breeding for粕ti—venicillium wilt． 1．T钾o F2 population derivcd fbm me intmspecific cross of the highly tole啪t (bssypillIn 11irsu劬n cv(xillluzhongl0)柚d the叭sc印tible G llirsut呦cv (Ju枷i柚1肋d xill】uzl】a07)w蜘ch ph锄哟pcd嚣non-def01iatjng V dall“ae聃惯．e吣ed ill present rese盯ch．By MAPMAKE舳Ⅻ(V酏ion3．ob)made use铆o liIll【age m印，the minIDD witll tIlrcc，tlle maxhcredity-dist锄ce witll 50 cM，the two蹦lctic linl【agc maps comprised of 156 loci cov删 1465．8 啪 geIlctic distallce’ 印proximately 26．65％of tlle whole cotton g吼ome．1wo di插溯lt disea辩outbreal【 peal【s of Venicilli啪wih d埘ng tl圯period of gIlitar bolls粕d wadding锄d secdling 印praisal we佗∞a皿ed by composite iIlterval mapping，with LDD thr鼯hold 2．5．Three QTLs related t0 rcsistallce werc detected∞tllrce diffb啪t lilll【age groupst 锄d 以pIailled 14．2‰48．4％of the仃ait V碰ati∞，indicating mat ttle msistance of VwiMs w船con仃Dlled by polyg吼es．1rt鹏e QTLs susc印tible to v—wilt wefe also detectcd锄d cxplained 11．3％、12．9％、45．2％oftI抢仃ait variati∞．Howev％all the three QTLs we陀liIll【ed with makcr NAU 1211，which indicated t11at“might be tlle s锄e locus． 2．QTLs for∞VcIl agronoIIlic锄ts，including first丘uit∞des，boll weight，lint pcrc锄tagc辩ed index，platlt hei曲t，main-10be 1cIl舀h锄d the second．10be were m印ped in觚o F2 p叩ulatiolls．Totally 17 stable QTb，one岛r fifst劬it nodes粕d explailled 7．O％ofthe trait V撕ation，t、Ⅳo for plant hei曲t and explained 7．5％，23．1％of the仃ait V撕ation，tll】他e f研seed indcx and explained 9．7％一l 8．8％ofthe仃ait v鲥ation。 f．oIIr forlim percImtage and explaine