蒙药珍宝丸对大鼠周围神经损伤修复作用研究-中(蒙)西医结合基础医学专业毕业论文.docxVIP

摘 摘 要 目的：本研通过究观察蒙药珍宝丸对大鼠坐骨神经挤压损伤的修复作用和珍 宝丸药物血清对雪旺细胞增殖的影响为珍宝丸治疗周围神经损伤方面提供理论 依据。 方法：选用成年SD大鼠，建立坐骨神经挤压损伤模型，将动物随机分成2组 对照组灌胃生理盐水，实验组灌胃珍宝丸混悬液，每组10K，灌胃‘3周，于术后2 周和4周进行坐骨神经功能指数测定，评价其功能。恢复情况。于术后4周，做神经 再生的组织学观察，评价神经再生情况。选用新生24hSD大鼠，取双侧坐骨神经， 体外培养并纯化。S-100免疫细胞化学染色法鉴定雪旺细胞，将纯化的雪旺细胞 分成4组培养，即胎牛血清组、高剂量含药血清组(1．259／kg)、低剂量含药血清 组(0．259／kg)、不含药血清组：于培养第l天、第一l天、第7天、lTT法观察各组雪 旺细『j包生长活性。 结果：术后2周和4周实验组大鼠SFI值均显岳一：≥。’二对照组，组织学观察显 示实验组的有髓神经纤维密度、髓鞘厚度郜高r《寸照组。s一1 0()免疫染色结果显 示纯化后的雪旺细胞纯度为9：3．隅。＼4TT法测得培养第7犬时-岛制毽含药血清组 0D值明显高于低剂量含药血清组和不含药血清组(JD值(尺()．05)，而全过秤中 胎牛血清组0D值明显高于不含药血清组()D值(只()．05)： 结论：体内实验：按1．259，。7kg给大鼠灌胃蒙药珍。土：丸对损伤的周围砷经的修 复有促进作嗣。体外培养SC。实验：一定剂量的含药血清对SC、增值有促进作用。 关键词：蒙药：中药：大鼠；周围神经：损伤 Effects Effects of Zhen Bao Wan on Repairation of Injured Peripheral Nerve in a Rat Abst ract Obiective：to test the effects of ZhenBaoWan on peripheral nerve regeneration． Methods：20 adult Sprague—Dawley rats weighing 2509+209 underwent unilateral(right)sciatic nerve crush．1．259／kg ZhenBaoWan administered orally to ten rats in experimental group three weeks，another ten rats in cintrol group received saline orally for three weeks．T11e animals were kept in standard room conditions and fed with standard rat diet and water ad libitum．14 and 30days after injury．functional and electromyography assessments of nerves were performed．Functional recovery was analyzed using a walking track assessment,and quantified using the sciatic functional index(SFI)．After these evaluations，all rats were sacrificed and microscopic evaluations were performed．the sciatic nerve from SD rats(24h)was cultured in vitro and purified．S．1 00 immunhistochemical staining were performed tO find the schwann cells．The purified Schwann cells were divided into 4 groups： normal control group、the different consistence rat s serum with drug and the rat s serum．MTT was used to observe the viability of the Schwann cells at different time． Results：nle sciatic functional index(SFI)in experimental group on 1 4 days after injury is above significently from control