抗枯萎病香蕉新品系农科一号选育及种性分子鉴定-生物化学与分子生物学专业毕业论文.docxVIP

!些奎兰堡主堡塞 !些奎兰堡主堡塞 抗枯萎病香蕉新品系农科一号选育及种性分子鉴定 年可在全省较大范围内推广种植。 利用ISSR分子标记技术从分子水平上分析农科一号与当前大面积推广种植 香蕉的亲缘关系，分析结果表明农科一号与当前大面积推广种植的巴西香蕉的亲 缘关系最近，遗传距离最小。 关键词：香蕉 品种选育香蕉枯萎病 分子标记 ISSR time)from time)from the introduced banana cultivars and varieties during the tissue culture of Brazil banana cultivar．Using the Brazil banana cultivar as control，we measured the capability of resistance to Banana’S Fusariam wilt．The results indicated that Nongke No．1 exhibited powerful resistance to Banana’S Fusariam wilt ability．While the control was infected by wounding roots only one time，it was not infected for Nongke No．1 until the fourth artificial wounding roots．The excellent ability to resist pathogen was also demonstrated by field plantation．The infection rate was only 5％一1 0％，much lower than ordinary cultivars that had a rate of 60％．90％． Rapid micropropagation of Nongke No．1 through tissue culture was established． 中山大学硕士论文 中山大学硕士论文 抗枯萎病香蕉新品系农科一号选育及种性分子鉴定 2006．3．29 Nongke No．1 differed from Brazil banana cultivar in adventitious bud induction， successive propagation and rooting：the stringent conditions were needed during tissue culture；the time of tissue culture should be short；the propagation index was low；adventitious buds and roots were short and strong． Nongke No．1 was similar to wide—planted Brazil banana in growth period，yield， fruit shape and eating quality etc．It is an ideal and new resistant line to Banana’S Fusariam wilt at the present and is favored by local peasants．They all ordered our plantlets after visiting our Nongke No．1 plantation．It is expected the planting area will reach 1 50 ha within Pearl River Delta at the end of 2006，and expand throughout the Guangdong Province in 2007． We also assessed the phylogenetic relationships of these banana cultivars at the molecular level using IS SR markers．Our results showed that Nongke No．1 has the closest relationships with Brazil bananas based on the smallest genetic distance． Key words：banana；cultivar breeding；Banana’S Fusariam w