黄连上清丸的药代动力学及血清药物化学研究-药物分析学专业毕业论文.docx

Ab Abstract PAGE PAGE IV Abstract Huang lian Shang Qing pills (HLSQP) which been incorporated in the 2010 edition of Chinese Pharmacopoeia is a widely used compound Chinese medicine for the treatment of clearing heat and relieving pain for five hundred years. It is composed of seventeen kinds of herbs, such as Rheum palmatum L, Coptidis rhizome, Scutellaria baicalensis, Cortex Phellodendri Chinensis and so on. However, the action mechanism and process and effective constituents of HLSQP in vivo is not clear and there were no any reports about the pharmacokinetic and serum pharmacochemistry studies of HLSQP. In order to control its quality and clarify pharmacodynamic material basis, a High Performance Liquid Chromatography （ HPLC ） method was created to simultaneously determinate the content of aloe-emodin and emodin in HLSQP and this is the first report for the study of pharmacokinetic and serum pharmacochemistry of HLSQP. A HPLC method was developed to determinate the content of aloe-emodin and emodin in HLSQP from different factories. Chromatographic separation was performed on an Inertsil?ODS-3 column (150mm×4.6mm, 5um) with a mobile phase consisting of methanol-0.2% phosphoric acid (65:35 v/v) at a ?ow rate of 1.0ml/min. Detection wavelength of analyte was set at 254 nm. The temperature of the analytical column was maintained at 25℃. The injection volume was 10 μl. The method was validated in terms of speci?city, precision, linearity and stability. Under this condition, aloe emodin in 14-41.40μg/ml（r=0.9999）and rhein in 6.49-29.70μg/ml（r=0.9998） exhibited good linearity of the calibration graphs. The results showed that content of aloe-emodin and emodin in HLSQP from partly different factories with different batch numbers was obvious different. A HPLC method with diode array detection was developed and validated to determinate rhein in rat plasma and its application to a pharmacokinetic study of HLSQP was investigated for the first time in