蒙药材漏芦花、荜茇、接骨木中黄酮类化合物的提取、测定与抗氧化性研究-有机化学专业毕业论文.docxVIP

摘 摘 要 本文对蒙药材研究发展状况，特别是蒙药材中黄酮类化合物研究状况，进行了简 述。黄酮类化合物的研究是蒙药研发的基础性研究。本课题选用蒙药中疗效甚好的常 用药漏芦花、荜茇、接骨木作为研究对象，借鉴中药研究黄酮类化合物的成熟方法， 采用超声波法对三种药材进行提取，并优化了提取条件；利用分光光度法测定其黄酮 含量：研究了其抗氧化性。研究表明：这三种蒙药材中黄酮类化合物含量较丰富，黄 酮类化合物提取液对羟自由基(·OH)、超氧阴离子自由基(02一．)、过氧化氢(H：02) 均有较强清除作用，并随着提取液浓度提高而呈现一定量效关系。本课题为研究这三 种蒙药材药理以及研制新型的药物提供了依据。 关键词：漏芦花；荜茇；接骨木；超声波法；抗氧化性；分光光度法 Extraction Extraction and Determination of Flavonoid from Rhaponticum uniflorum ，Piperlongumi，Sambucus williamsii Hance and their Antioxidition Activity Abst ract The article briefly summarizes the development of Mongolian medicine，especially the research of flavanoids．The study of flavanoids is the foundation of research and development in Mongolian med．icine．The art／de chooses some commonly used medicine which have good effects such rhaponticum uninorum,piperlonguml,sambucus williamsii hance the main objects of study,at the same time We also take advantage of Chinesc traditional medicines to study the mature method of flavanoids．We supersonic method tO extract three kinds of medicines and opfimizate the extractal condition；We deploy spectrophotO metric method tO measure the amount of flavanoid．we study the antioxidition activity of three kinds of medicines．The result shows the flavanoids extract of the three kinds of mongolian medicines have s灯ong effect the clearance of hydmxy radical， superoxide anion freeradical，hydrogen dioxide and with the consistency of extract increase，it presents certa／n dose-cffect relationship．The topic lays the foundation of studying the three kinds of mongolian medicines well developing new kinds of medicines further． Key wo rd s：Rhaponticum uniflorum、Piperlonguml、Sambucus williamsii hance； supersonic method；Antioxidition Activity；spectrophotometric method 插图和附表清单1．图l 插图和附表清单 1．图l 乙醇浓度对漏芦花提取率的影响 8 2．图2超声时间对漏芦花提取率的影响 9 3．图3物料比对漏芦花提取率的影响 ．．10 4．图4漏芦花黄酮类化合物提取液对羟自由基(·伽)清除效果 1l 5．图5漏芦花黄酮类化合物提取液对超氧阴离子自由基(02一．)清除效果 ．12 6．图6漏芦花黄酮类化合物提取液对过氧化氢(11202)清除效果 12 7．图7乙醇浓度对荜茇提取率的影响 ．．14 8．图8超声时间对荜茇提取率的影响 ．．14 9．图9物料比对荜茇提取率的影响