基于Plackett–Burma的湖南芥菜发酵菌种高密度培养条件优化.PDFVIP

() 2017, 43(6):669–675. DOI:10.13331/ki.jhau.2017.06.017 Journal of Hunan Agricultural University (Natural Sciences)  基于 Plackett–Burma 的湖南芥菜发酵菌种 高密度培养条件优化 * ( 410028)   摘 要 LA R6 R2 Plackett–Burma 11 LA R6 R2 (Plackett–Burma) LA R6 3%1.5%0.5%1%pH 6.0 3%33 ℃21 h3.62×1010 cfu/mL R2 4% 1.5%0.5%2%pH 6.03%29 ℃ 21 h1.85×1012 cfu/mL 关 键 词 中图分类号：TS252.53 文献标志码：A 文章编号：1007− 1032(2017)06−0669−07 Optimizing the high–density culture conditions for bacteria fermentation of chinese leaf mustard based on Plackett–Burman * HOU Aixiang, WANG Yiqi, JIANG Hui, ZHENG Xu, LI Zongjun (College of Food Science and Technology, Hunan Agricultural University, Changsha 410028, China)   Abstract: In order to obtain high–density cultivation condition for the fermentation of Hunan brassica juncea, mixed strains of LA and R6, and single strain R2 which were suitable for the mustard starter culture, were screened out in the natural fermentation process based on indicators of sensory evaluation and nitrite content. 11 kinds of nutrients including glucose, ammonium sulfate, tryptone and beef extract were screened out as the main proliferation substances for mixed strains of LA and R6 according the result of Plackett–Burma design method; while whey powder, ammonium sulfate, tryptone and yeast extract powder were screened out for those of strain R2. The results showed that the optimum formula for mixed culture medium of LA and R6 was consisted of 3% glucose, 1.5% beef extract, 0.5% ammonium sulfate, and