裸小鼠人胃癌肝转移模型建立及两种方法的比较-外科学(普通外科学)专业毕业论文.docxVIP

新疆医科大学硕士学位论文 新疆医科大学硕士学位论文 PAGE PAGE 1 裸小鼠人胃癌肝转移模型建立及两种方法的比较 导师：王云海 教授 研究生：马玉龙 摘 要 目的：胃癌是临床常见的恶性肿瘤之一，侵袭、转移和复发是影响胃癌患者预 后的主要因素。目前胃癌肝转移是胃癌根治术后患者死亡的最主要因素。现阶段胃 癌肝转移的原理及机制尚不清楚。为进一步研究胃癌肝转移机制，建立理想的动物 肿瘤移植模型是进行肿瘤防治研究的重要前提。建立人胃癌裸小鼠肝转移模型，及 两种不同建模方法的比较。从而为进一步明确胃癌肝转移机制，提供动态观察模型。 方法：将肿瘤细胞悬液种植于裸小鼠腹腔形成肝转移和将肿瘤组织块原位种植于裸 小鼠胃壁形成原位移植瘤继而肝转移，观察和比较了两种方法所建立模型原位肿瘤 生长状况、移植成功率和自发转移的发生率。结果：胃癌组织块种植的原位成瘤率 达 100％，淋巴结广泛转移率 66％，肝转移发生率 75％。肿瘤细胞悬液种植于裸小 鼠腹腔，胃原位癌生长率 33%，淋巴结广泛转移率为 50％，肝转移发生率仅为 25 ％。结论：两种移植和转移模型均可形成肝转移特点，以肿瘤组织块原位模型为优， 该模型的建立可为人胃癌转移机制和人胃癌肝转移后相关因子测定提供一种有价值 的工具。 关键词：胃肿瘤； 胃癌； 肝转移模型； 裸小鼠 Establishment Of Two-orthotopic Implant-metastatic Models of Human Stomach Cancer in Nude Mice Postgraduate：YuLong Ma Supervisor：Professor Yun-hai Wang Abstract Objective: To develop two methods of orthotopic implant-metastatic models of Human gastric cancer in nude mice and to compare both methods of two models. Methods: Intact tumor tissue or lumor cell suspension was orthotopically implangted into the nude mice stomach to develop implant-tumors. The tumor growth characteristics, tumortake rates and metastatic rates of the two motels were analyzed and compared. Results: Tumor-take rate was 100% in the motel implanted with inpact tumor tissue. Inthis motel, the metastases of lymph nodes and livers were 75% and 25% respectively. In the metel implanted with tumer cell suspension, the local tumor growth was slower, tumor-take rate was 67%. The metastases of lymph nodes and livers were only 66% and 50% respectively. Conclusion: There is growth characterstics of human stomach cancer in both orthotopic tumor models. The model implanted with intact tumor tissue is better than another. This tumor model might be a useful tool in research about metastatic mechanisms and treatment of anti-metastasis in human stomach cancer. Keywords: stomach neoplasms; tumor cell; disease; nude mouse; 中英文缩略词对照表 英文缩写 英文全名 中文译名 NM Nude Mice 裸鼠 BM basemembrane 基底膜 c-Met Hepatocyte growth factor acceptor  肝细胞生长因子受体 EGF epidermal growth