黑麦草玉米谷蛋白水解产物中分离的一种五肽的生物活性资料.pptVIP

黑麦草玉米谷蛋白水解产物中分离的一种五肽的生物活性 bioactivity Corn gluten meal (CGM) is a byproduct of corn (Zea mays L.) from the wet-milling process and has been patented as a natural preemergence herbicide. It inhibits root growth of germinating seeds but does not damage plants that have formed a mature root system. Because CGM contains approximately 10% nitrogen by weight, it is useful as a natural preemergence herbicide and fertilizer for various plant production systems. Corn gluten hydrolysate (CGH), a water-soluble material derived from the action of bacterial proteinase, was found more herbicidally active against three grass species than CGM both in the soil and Petri dish bioassays. It also has a greater nitrogen content and could potentially be used to fortify the CGM to improve it as a natural weed and feed material . Its high water solubility makes it sprayable and easy to apply as a herbicide in some crop production systems. It is also useful as a source for the isolation and identification of the bioactive compounds. Materials and Methods Perennial Ryegrass Bioassays after 7 days: The longest root and shoot of each seedling were measured. 7 measurements found to have root lengths within ±1S.D The seven measurements average root length of the control （received D.D. H2Oonly） The value was expressed as the percentage of the control length. Extraction and Purification Gluten hydrolysate powder Sephadex G-15 gel filtration column: calibrated with blue dextran and NaCl aqueous solution eluted with D.D. H20 at a flow rate of 1.6 mL/min. a Gilson model 201 fraction collector 30 ml GH-f was loaded onto the column 12 mg of 53GH-g15-e 12 mg of the bioactive 53GH-g15-e was injected onto a semipreparative Delta Pak with a MeOH in water linear gradient of from 0 to 15% in 30 min at a flo