艾迪注射液对食管癌凋亡影响的组织芯片研究.docxVIP

艾迪注射液对食管癌凋亡影响的组织芯片研究 【摘要】 目的 研究艾迪注射液对食管癌凋亡的影响。方法 收集食管鳞状细胞癌病人46例，对照组23例，艾迪组23例，艾迪组术前给予艾迪注射液80ml/d，静脉滴入，连用7天，对照组不用，两组术前其他处理相同。术后完成常规病理检查后，应用组织芯片技术，采用免疫组化法检测Bcl-2、Bax；原位末端核苷酸标记(TUNEL)法检测癌细胞凋亡状况。结果 两组在性别、年龄、部位、癌肿大小、分化、浸润深度、淋巴转移方面差异均无显著性，具有可比性。 Bcl-2在艾迪组的表达阳性率比对照组显著降低，具有统计学差异，Bax在艾迪组的表达阳性率比对照组显著增高，具有统计学差异。艾迪组的Bax/ Bcl-2比值(±）比对照组增高，差异有非常显著性。TUNEL法检测：艾迪组癌细胞凋亡阳性率比对照组明显增高，两组间差异有非常显著性。结论 艾迪注射液可以通过下调Bcl-2蛋白的阳性表达，增大Bax/ Bcl-2的比值、诱导癌细胞凋亡，从而发挥对食管癌的术前干预作用。应用组织芯片大规模高效检测临床组织样本是可行的，具有快速、 经济 、准确的特点。  【关键词】 艾迪注射液; 食管鳞状细胞癌; 组织芯片; 凋亡   Study on the influence of apoptosis in Aidi injection on patients with esophageal squamous cell carcinoma(ESCC) by tissue microarray  WANG Jian-rong,LAI Medical College of Nanjing University of TCM,Affiliated Hospital of Nanjing University of TCM , Nanjing10029,China  【Abstract】 Objective To study the influence of apoptosis in Aidi injection on patients with ESCC. MethodsESCC patients were divided into Aidi group and control group, each of which includedsubjects. Patients of Aidi group were given0ml/d intravenous injection of Aidi for days before surgery, while patients of control group were not given any Aidi injection. Other related therapeutic medicines were as same in both groups. After completing ordinary staining detection, tissue microarray (TMA) was prepared and performed using immunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated dUTP biotin end labeling of fragmented DNA(TUNEL) method. Bcl-and Bax were observed by immunohistochemistry staining. Results There was no significant statistical difference between the two groups in sex, age, tumor sites, tumor size, differentiated degree, infiltration degree or lymph node metastasis. The positive rate of Bcl-in Aidi groupwas significantly lower than those in control group,,while the rate of Bax in Aidi groupwas significantly higher than that in control one,. The ratio of Bax/ Bcl-in Aidi group (±) was significantly higher than that in control g