毕业论文：《锦香梨基因组DNA提取的比较研究》.docVIP

精品论文 第 PAGE 17 页 共 17页 精品论文 锦香梨基因组DNA提取的比较研究 摘要 本实验以锦香梨的叶片和种子为材料，采用SDS法和CTAB法分别对其基因组DNA进行提取。结果表明，不同组合提取锦香梨的基因组DNA在纯度及产量上均存在差异。DNA提取的纯度从高到低依次为：CTAB法提取叶片中基因组DNA(OD260/OD280=1.3688)、CTAB法提取种子中基因组DNA (OD260/OD280=1.1777)、SDS法提取叶片中基因组DNA(OD260/OD280=1.1368)、SDS法提取种子中基因组DNA (OD260/OD280=1.1212)；所得DNA平均含量从大到小为：SDS法提取种子中基因组DNA(OD260 =0.370)、SDS法提取叶片中基因组DNA(OD260 =0.367)、CTAB法提取种子中基因组DNA( OD260 =0.242)、CTAB法提取叶片中基因组DNA(OD260 =0.141)。综合分析，采用CTAB法从锦香梨叶片中提取基因组DNA为最佳方法。 关键词：锦香梨；基因组DNA；提取 COMPARATIVE STUDY OF GENOMIC DNA’S EXTRACTION OF JIN FRARANT PEARS ABSTRACT Leaves and seeds of Jin fragrant pears are the materials of this experiment, take SDS method and CTAB method to extract their genomic DNA respectively. Results showed that there are some differences in Genomic DNA of extracting by different combination in purity and output. The purity of DNA extraction ,from high to low, is CTAB method extracting genomic DNA from leaves(OD260/OD280=1.3688),CTAB method extracting genomic DNA from seeds(OD260/OD280= 1.1777),SDS method extracting genomic DNA from leaves(OD260/OD280=1.1368),SDS method extracting genomic DNA form seeds(OD260/OD280=1.1212). The average content of DNA extraction, from high to low, is SDS method extracting genomic DNA from seeds(OD260 =0.370) ,SDS method extracting genomic DNA from leaves (OD260 =0.367)，CTAB method extracting genomic DNA from seeds ( OD260 =0.242), CTAB method extracting genomic DNA from leaves(OD260 =0.141).Comprehensive analysis CTAB method extracting genomic DNA from leaves is the best method. Key words：Jin fragrant pears; Genomic DNA; Extraction 目录 TOC \o 1-3 \h \z \u 1 前言 1 1.1 锦香梨简介 1 1.2 DNA简介 1 1.3 常用的基因组DNA含量及纯度测定方法 2 1.4 目前国内外梨属植物基因组DNA提取的研究状况 2 2 材料与方法 4 2.1 材料 4 2.2 试剂 4 2.3 试剂的配制 5 2.3.1 2×CTAB提取液的配制 5 2.3.2 1×CTAB沉淀液的配制 5 2.3.3 10%CTAB的配制 5 2.3.4 TE缓冲液的配制 6 2.3.5 SDS提取液 6 2.4 仪器 6 2.5 方法 7 2.5.1 CTAB 法 7 2.5.2 SDS法 8 3 结果与分析 9 3.1不同提取方法及提取部位提取产物测