油菜蜜中硫代葡萄糖甙及其降解产物的鉴定.PDF

※分析检测 食品科学 2009, Vol. 30, No. 20 363 油菜蜜中硫代葡萄糖甙及其降解产物的鉴定 1 1, 1 2 张红城 ，董 捷 * ，张 旭 ，高 远 ( 1.中国农业科学院蜜蜂研究所，农业部国家蜂产品加工专业分中心，北京 100093 ； 2.南京农业大学食品科技学院，江苏南京 2 10095) 摘 要：对油菜蜜中的硫代葡萄糖甙及其降解产物进行提取与定性分析。通过有机溶剂对油菜蜜中的硫代葡萄糖 甙及其降解产物进行萃取，然后进行氯化钯显色反应，并采用气相色谱- 质谱联用技术对硫代葡萄糖甙及其降解产 物进行分离鉴定。结果表明新鲜的油菜蜜含有硫代葡萄糖甙，放置半年后油菜蜜中含有硫代葡萄糖甙的降解产物—— 异硫氰酸酯。随储藏时间的延长，新鲜的油菜蜜中的硫代葡萄糖甙会降解成异硫氰酸酯。 关键词：油菜蜜；硫代葡萄糖甙；异硫氰酸酯；降解产物；鉴定 Identification of Glucosinolate and Its Degradation Product in Honey of Brassica nap us L. 1 1, 1 2 ZHANG Hong-ch en g ，DONG Jie * ，ZHANG Xu ，GA O Yuan (1. Institute of Apicultural Research, Chinese Academy of Agricultural Science, National Research Centre of Bee Product Processing, Ministry of Agriculture, Beij ing 100093, China ；2. College of Food Science and Technology, Nanj ing Agricultural University, Nanj ing 2 10095, China) Abstract：Glucosinolate and its degradation products in honey of Brassica nap us L. were extracted using mixed organic solvents. These obtained products were subj ected to quantitative examinations by palladium chloride method and further isolated and identified by gas chromatography-mass spectrometry (GC-MS). Results revealed that glucosinolate was ob served in fresh honey of Brassica nap us L., and isothiocyanate, one of degradation products of glucosinolate was detected in honey of Brassica nap us L. during storage for half year at room temperature. Moreover, more glucosinolate in fresh honey of Brassica nap us L. were degraded to form isothiocyanate with prolongation of storage. Key words ：honey of Brassica nap us L ；glucosinolate；iso