- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
The
The Laser Microdissection system of Chinese fir and Popuplus
cambium and RNA quality control
Abstract
Laser mierodissection(LMD)is a technique which can isolate specific tissues,cell types and eveu organeHes from sectioned biological specimen under the microscope in a manlier conducive to the extraction of RNA.DNA or protein.Pioneering research has demonstrated the
potential of LMD as a powerful tool for investigating molecular and biochemical events that
occur in discrete cells ofcomplex,heterogeneous tissues。with the characteristic offast,precise and easy-operation.LMD,which is an established technique in many areas of biology,has now been successfully adapted for use wi血plant tissues.
Woody plants have the ability to produce secondary tissue(secondary xylem and phloem)from a medstematic tissue called vascular cambium,responsible for the radial growth of a tree.The research ofexpression and control ofgene during the wood formation by molecular and cytological is an important scientific issue for scientists.However,the knowledge of cambium is limited.because it is hard to separate the cambium ceIls from other surrounding cells by traditional methods.Selecting the cambium cells precisely is a key point for the research of
cambium cells.Since 1990s,the development of Laser Microdissection facilitated the collection
of special materials.
In the first step of research,we established a suitable cryo—sectioning method for stem organs of Chinese fir and Populus.With studying on the technical parameters of fixation and pretreatment Of tissue.cultured material.a suitable procedure consisted of several steps is considered the best protocol as follows.The samples were first fixed in Canoy’s fixation for 4 to 6 hours,and then
saturated in 10%sucrose PBS solution for 6 to 12 hours.After embedded they were frozen in
fiquid nitrogen for 50 to 90 seconds and cryo·sectioned.The procedure of dehydration after cryo—sectioning improved the quality of section to a certain extent.T
您可能关注的文档
- 论企业社会责任的概念在舶来过程中的蜕变-经济法学专业论文.docx
- 论民事恶意诉讼-诉讼法学专业论文.docx
- 山东方言声调研究-汉语言文字学专业论文.docx
- 利用瞬态吸收光谱技术研究水相中·OH自由基与苯及其衍生物的微观反应机理-环境科学专业论文.docx
- 昆山市社区卫生服务“十一五”进展评价-公共卫生专业论文.docx
- 论海南农垦改革中的非胶农地使用制度-经济法学专业论文.docx
- 卵形消化池流-固耦合系统的ADINA有限元分析-结构工程专业论文.docx
- 利用扫描电镜观测聚乙烯基纳米电介质微结构方法的研究-高电压与绝缘技术专业论文.docx
- 辽河常渣中镍化合物的分离与鉴定-化学工程与技术专业论文.docx
- 利用半夏凝集素构建内生工程菌及其抗同翅目害虫效果研究-微生物学专业论文.docx
最近下载
- 2025年西师版三年级上册数学全册教案(完整) .pdf VIP
- 土木工程学报论文模板.docx
- 肝功能化验单解读课件.pptx VIP
- 生产部部门和岗位职责.doc VIP
- 踝泵运动【优质公开课】精品PPT课件模板.pptx VIP
- 2023年咸阳经开城市发展集团有限公司人员招聘考试参考题库及答案解析.docx VIP
- 医院物业保洁保安投标服务方案(技术方案).doc
- 第8课+建设法治中国(课时1)(实用课件)-【中职专用】中职思想政治《职业道德与法治》高效课堂精品实用课件+教案(高教版2023·基础模块).pptx VIP
- 教学设备采购投标方案(技术方案).doc
- 余姚中学2008年自主招生考试数学试题.doc VIP
文档评论(0)